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1

Mechanical damage of animal cells in bioreactors

100%
Jankowski T., Grajek W.
Biotechnologia
|
1993
|
issue 3
166-183
EN
of animal cells due to forces generated by media agitation and aeration are reviewed. In anchorage-dependent cultures grown in a stirred bioreactor the cell damage is caused by small turbulent eddies of size of the microcarrier beads and by collision between and against the impeller and the stationary parts of bioreactor. In the freely suspended cells grown in stirred or s the cell damage is due mainly to air bibble breakup. The mechanical damages can be limited by an increase of kinematic viscosity of fluid and reduction to the local energy dissipation rate. Biological aspects of shear stress are also discussed.
2

Problems in design and operation of bioreactors for plant and animal cell cultures

100%
Pohorecki R.
Biotechnologia
|
1996
|
issue 2
125-135
EN
Basic modes of bioreactor operation (bath, semicontinuous and continuous), and main culture systems (suspended growth, attached growth on microcarriers) are shortly described.Main problem in bioreactor design and operation, including hydrodynamics, heat and mass transfer, cell viability, measurement and control, are discussed.Some particular problems: mixing systems, air supply systems, power dissipation, external and internal diffusion are discussed in a greater detail.Examples of specific reactor design are given, namely: stirred tank reactors, bubble columns, gas lift reactors, liquid jet reactors, packed bed reactors fluidised bed reactors, membrane reactors.New research problem are outlined.
3

Pilot plant and industrial systems for animal cell cultivation.I.Anchorage-dependent cell cultures

100%
Grajek W., Lukaszynski D.
Biotechnologia
|
1993
|
issue 2
8-21
EN
Several methods used to cultivate anchorage-dependent animal cells on a large scale are presented. These methods include the cultures on microcarriers in stirred and air-lift bioreactors, the cultures in hollow-fiber and flat-plate membrane bioreactors as well as the cultures attached to porous or fiber solid supports. The critical parameter in anchorage-dependent cultures is the adhesiveness of the cells to the carrier materials and the surface proliferation rate.
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