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1

Serotonin increases the phase shift of the circadian locomotor activity rhythm in mice after dark pulses in constant light conditions

100%
Bartoszewicz R., Barbacka-Surowiak G.
Folia Biologica
|
2007
|
vol. 55
|
issue 3-4
101-108
EN
Investigations on the effects of the 5-HT agonists and antagonists on the phase of the circadian locomotor activity rhythm of animals kept in constant light conditions (LL) are rare. Therefore the influence of R-(+)-OH-DPAT (5-HT1A receptors agonist) and metergoline (5-HT1/2/7 receptors antagonist) on the phase shift of the locomotor-activity rhythm alone and when combined with dark pulses in mice kept in LL are examined. The results indicate that 8-OH-DPAT administered independently at 12.00CT (Circadian Time) shifted the phase of the circadian rhythm and reinforced the effect of dark pulses on this parameter. 12.00CT was defined arbitrarily as the onset of locomotor activity in constant conditions. Metergoline diminished the phase shifts after dark pulses compared to 8-OH-DPAT. The influence of the serotonin agonist showed that serotonin can reinforce the phase shifting effect of the locomotor activity rhythm after dark pulses in LL condition.
2

Tools of the trade: diagnostics and research in domestic animal cytogenetics

100%
Iannuzzi L., Berardino_Di D.
Journal of Applied Genetics
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2008
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vol. 49
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issue 4
357-366
EN
This paper describes the most common cytogenetic techniques we routinely adopt in our laboratories for producing high-resolution banding on prometaphase stage chromosomes, from synchronized or nonsynchronized blood cultures. Special emphasis is given to the FISH procedures applied to prometaphase chromosomes for mapping purposes. Each section includes historical information, basic principles for the given technique, its primary use in veterinary cytogenetics, and major limitations. Supplementary material (protocols and chemicals used) are available on our website. Even though these techniques mainly refer to the Bovidae, they can be easily extended and adapted to members of other taxa.
3

The role of galanin in physiological and pathological processes in a human body

86%
Zdrojewicz Z., Sowinska E.
Postępy Higieny i Medycyny Doświadczalnej
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2000
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vol. 54
|
issue 6
819-834
EN
Galanin (GAL) a 29 amino-acid peptide, is distributed in the central and peripheral nervous system, the pituitary gland the gastrointestinal tract and also in the endocrine and exocrine pancreas. The endogenous and exogenous effects of galanin are mediated by three receptor subtypes, which are termed: GALR1, GALR2, GALR3. Galanin has a significant role in physiological and pathological processes (acromegally, diarhoea, collitis, Alzheimer?s disease, oberitas depression, pituitary gland adenomas) in a human body and animals. It has an ability to contract smooth muscles in gastrointestinal tract, stimulates reflexes in the central nervous system, decreases pancreatic amylase, release changes transport of elektrolytes Na+ and Cl-, exerts tonic inhibition of nociceptive input to the central nervous system, stimulates glucagon release, inhibits insulin and somatostatin release, takes part in prolactin secretion, stimulates growth hormone ? releasing hormone, hypothalamic gonadotropin releasing hormone and corticotropinreleasing hormone. It causes increase of somatotropin secretion,foliculotropin and luteinizing hormone release and adrenocorticotropin secretion.
4

Controversies over the evaluation of health safety of products of plants transgenesis

86%
Zdunczyk Z., Jankowski J.
Biotechnologia
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2009
|
issue 3
9-21
EN
The paper provides an overview of the most important in vivo experiments, concerning herbicide tolerant soybean and Bt maize, quoted by the opponents of GMOs, as to justify opposition to the use of GM crops in animal feed and food production. The results of these experiments were confronted with similar studies performed by other research teams. Relatively few in vivo experiments have demonstrated significant differences in selected parameters of metabolic and health status of animals fed diets with soya or GM maize diets. Concluding on that basis that HT soybean and Bt maize may pose health threat to animals and humans is not eligible.
5

Application of reproduction biotechnology methods in animal biodiversity conservation

86%
Gajda B., Smorag Z.
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2007
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issue 4
55-65
EN
This article presents the potential and prospects for the use of selected reproduction biotechnology methods in animal biodiversity conservation programs. The first part focuses on biotechnological methods related to male reproduction such as artificial insemination, semen cryoconservation, sexing and spermatozoa sorting. The second part discusses biotechnological methods related to female reproduction such as superovulation, in vitro production and transfer of embryos, cryoconservation of oocytes and embryos, embryo sexing, cloning and transgenesis.
6

Actinomycetes in nature and biotechnology

86%
Mordarska H., Piasciak M.
Biotechnologia
|
2002
|
issue 4
142-164
EN
This article summarizes the medical, ecological, industrial and biotechnological importance of actinomycetes. At present, according to a new hierarchic classification system, they are the most representative group of Actinobacteria classis nov. These unusual microorganisms with such rich biological activity have had a confusing history and classification for over a hundred years. In this review, following subjects have been raised: important, but less known human, animal and plant pathogens as well as actinomycete-diseases; symbionts as endophytes in nitrogenase activity; agents of biological control; biodeterioration and biodegradation; primary and secondary metabolites including their biological, industrial and biotechnological importance. Additionally, some lipid compounds of cell wall as immunomodifiers have been also mentioned. Actinomycetic activities have always had far-reaching effects, and these are sure to remain in different guises.
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