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  1. 1 Journal of Applied Genetics

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  1. 1 1995

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  1. 1 Strzyzewska C.

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Amphidiploid hybrids of Trifolium pratense L. (2n=14+2) with T.diffusum Ehrh. (2n=16)

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Strzyzewska C.
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After duplicating the chromosome number by colchicine in sterile F1 16-chromosome hybrid T.pratense x T. diffusum some partially fertile plants with 32 chromosomes were found.Male fertility (viability of pollen grain) was from 69.3% to 86.2% , whereas female fertility estimated as seed setting after cross-and self-pollination was 21.8% and 6.9%, resp.Male and female fertility as well as somatic chromosome number were examined in the F2-F4 generations.Selection for female fertility resulted in increasing seed setting in the first two generations (F2 and F3) and in decreasing it in F4 generation.An average seed setting in the F2-F4 generations after cross-pollination amounted to 22.2%, 43.6% and 12.9%, respectvely; after self-pollination it was 25.2%, 27.6% and 1.9%.n the F2 generation all the plants had 2n=32 chromosomes.In the next generations there appeared aneuploids, among which 30-chromosome individuals were predominant.
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