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Influence of dendritic cells on the in vitro allogeneic cytotoxic reaction of lymphoid cells derived from normal or listeria innocua-infected BALB/c mice

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Goscicka T., Walencka M., Fol M., Krupa A., Szeliga J.

Archivum Immunologiae et Therapiae Experimentalis

2001

vol. 49

issue 6

447-452

The role of lymphoid dendritic cells (DCs) in the development of an allogeneic cytotoxic reaction in vitro was examined. The T+B and T cell subsets originating from the spleens or lymph nodes of normal and Listeria innocua-infected BALB/c mice were used as the effector cells. Their cytotoxicity to 51Cr-labeled C3H fibroblasts was determined after removal of DCs and replacing them again. Moreover, the influence of exogenous mrIL-12 on the potency of DCs in the allogeneic reaction developed in vitro was checked. It was found that the DC-deprived T+B or T subsets of splenocytes, regardless of their origin, exhibited 27-38% lower cytotoxicity than those accompanied by natural DCs. The cytotoxicity of these subsets from normal lymph nodes decreased by 22%, while the activity of bacteria-primed cells dropped by 38%. Replenishing effector cells with isolated DCs restored their cytotoxicity. Pulsation of normal DCs with IL-12 had no effect on the recovery of normal cell cytotoxicity. However, the IL-12-pulsed DCs were able to intensify the cytotoxicity of T+B subsets derived from the spleens or lymph nodes of L. innocua-infected mice. The results suggest that the alloantigen presentation by DCs to cytotoxic lymphocytes also takes place in the reaction developed in vitro, regardless of effector cell origin.

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1 Archivum Immunologiae et Therapiae Experimentalis

1 2001

1 Fol M.

1 Goscicka T.

1 Krupa A.

1 Szeliga J.

1 Walencka M.

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Influence of dendritic cells on the in vitro allogeneic cytotoxic reaction of lymphoid cells derived from normal or listeria innocua-infected BALB/c mice