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1

The influence of DNA isolation's method on result of genetic similarity analysis made by RAPD technique

100%
Dobrzycka A., Broda Z.
Biotechnologia
|
2009
|
issue 3
182-191
EN
The study purpose was to examine the influence of DNA isolation's method on result of genetic similarity analysis made by RAPD technique. Two methods od DNA isolation were used ? the Tomson-Henry method, which provides low purified DNA, and the column method, which provides high purified DNA. By using DNA isolated with these two methods the RAPD analysis of genetic distance was made. It was noticed, that using high purified DNA resulted in higher nu umber of amplification's products, and consequently in larger accuracy of analysis.
2

Correlations and heritability of the characters determining the seed yield of the long-raceme alfalfa (Medicago sativa L.)

88%
Bodzon Z.
Journal of Applied Genetics
|
2004
|
vol. 45
|
issue 1
49-59
EN
Phenotypic correlation coefficients and heritability of the characters controlling seed yield of long-raceme forms of alfalfa was determined. It was found that seed yield per plant, which was positively correlated with 10 out of 12 analysed characters, depended upon the number of pods per raceme and the number of seeds per pod. Variability of these characters determined about 60% of the variability of seed yield. Multiple linear regression and phenotypic correlations show that simultaneous selection for increased pod number per raceme and increased seed number per pod and raceme length resulted in enhanced seed yield potential. The share of the additive genetic effects in the phenotypic variance for number of pods per raceme was low and about 21-23%, while for number of seeds per pod and per raceme amounted to about 50%. The expected genetic progress in recombination breeding for number of seeds per pod and number of seeds per raceme will be of medium magnitude, while one cannot expect any rapid and considerable progress in the number of pods per raceme. Considering the high positive correlation between raceme length and number of pods and seeds per raceme, one should conclude that raceme length can be an important criterion in selection of plants showing a high seed productivity.
3

Alfalfa (Medicago sativa) as an object for transformation by Agrobacterium tumefaciens and in vitro regeneration

75%
Lisova O., Legocki A. B., Legocki A. B.
Biotechnologia
|
2002
|
issue 3
74-90
EN
From the economical point of view alfalfa is a very valuable plant. It has very high yield potential, it is rich in protein, vitamins and minerals, so it is prized as very important feed for horses, beef cattle, sheep, goats and other domestic animals. That is why the improving of alfalfa quality by the methods of molecular biotechnology is a very interesting issue. In this review information about the crucial elements of alfalfa transformation and regeneration are gathered together. Among other following elements, genetic background of plant, A. tumefaciens strains, explant types, in vitro culture medias, plant development stages are discussed. It seems that optimalisation of the transformation and regeneration procedure are individual for every plant genotype and A. tumefaciens strain.
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