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1

Quantification of neutral red pinocytosis by small numbers of adherent cells: comparative studies

100%
Plytycz B., Rozanowska M., Seljelid R.
Folia Biologica
|
1992
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vol. 40
|
issue 1-2
3-9
EN
A colorimetric assay has been developed for studies on neutral red (NR) pinocytosis by small numbers of adherent cells cultured in 96 well plates. The NR uptake per cell mass was much higher in the sea urchin perivisceral adherent cells and human HL-60 cell line monolayers than in the nurine and Atlantic salmon macrophages. The apparent difference points to the usefulness of this novel assay in comparative studies.
2

Matrix metalloproteinase and cytokine production by bone marrow adherent cells from multiple myeloma patients

75%
Zdzisinska B., Walter-Croneck A., Dmoszynska A., Kandefer-Szerszen M.
Archivum Immunologiae et Therapiae Experimentalis
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2006
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vol. 54
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issue 4
289-296
EN
Introduction: Cultures of bone marrow stromal cells derived from the bone marrow of multiple myeloma (MM) patients were shown to exhibit several abnormalities compared with control cultures from healthy subjects. The aim of the study was to examine whether cultures of bone marrow adherent cells, at low passage level, exhibit differences in matrix metalloproteinases (MMPs) and cytokine production compared with cultures from normal donors. Materials and Methods: MMP production was evaluated by gel zymography and by ELISA in supernatants of serum-free cultures of bone marrow adherent cells derived from 20 MM patients and 23 healthy controls. Spontaneous and lipopolysaccharide (LPS)- or Newcastle disease virus (NDV)-induced cytokine release was assessed in the supernatants of the cultures by the ELISA method. Results: Both cultures produced MMP-1, -2, -3, and -9 under serum-free conditions; however, the levels of MMP-1 and MMP-2 were significantly higher in cultures derived from MM patients, while MMP-3 was significantly higher in control cultures. The level of MMP-9 was comparable in the cultures derived from MM patients and controls. All cultures produced interleukin (IL)-10 and IL-11 spontaneously, but after LPS or NDV induction the levels of IL-10, IL-11, interferon , and tumor necrosis factor , were significantly higher in the cultures derived from MM patients than in control cultures. Conclusions: The results indicate that both the abnormalities in MMP production and the overproduction of cytokines (in the presence of LPS or virus, which mimic inflammatory conditions) may be involved in bone destruction and tumor spread in multiple myeloma.
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