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1

Leukocyte acid phosphatase and metabolic efficiency of phagocytes in the first lactation trimester of cows from a leukaemic herd

100%

Kaczmarczyk E., Bojarojc-Nosowicz B., Fiedorowicz A.

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EN

In Black-and-White cattle, polymorphism of acid phosphatase (AcP) of blood leukocytes is determined by a pair of autosomal alleles. The aim of the study was to determine the relationship between AcP polymorphism and the metabolic efficiency of phagocytes in the first months after calving of cows naturally infected with the bovine leukaemia virus. The studied population consisted of 91 Black-and-White cows aged 3?6 years, from one herd. Enzootic bovine leukaemia (EBL) was diagnosed with the immuno-enzymatic ELISA method and a PCR molecular test. Additionally, agarose gel electrophoresis and the cytochemical method were used to determine the AcP polymorphism and activity in leukocytes. The metabolic activity of phagocytes was determined by the nitroblue tetrazolium (NBT) reduction test. Significant differences in metabolic efficiency of granulocytes were observed between cows representing different AcP phenotypes. No significant differences in levels of the analysed indices were observed between the EBL-positive and EBL-negative cows and between the three subsequent months after calving.

2

Two-dimensional electrophoresis patterns of total proteins, esterases and acid phosphatases from pollen, seeds and leaves of three cultivars of Helianthus annuus L.

88%

Kalinowski A., Klimko M., He L., Kluza M.

Journal of Applied Genetics

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2002

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vol. 43

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issue 4

437-450

EN

Total proteins, esterases and acid phosphatases from pollen, seeds and leaves of three sunflower cultivars were separated by 2-D electrophoresis. The characteristic peptides for each cultivar were identified. The seeds and pollen of the cultivar Wielkopolski contained 45 and 37 characteristic peptides, respectively, while the seeds and pollen of Coril contained 73 and 35 characteristic peptides. The cultivar Frankasol had the lowest total number of stained peptides in seeds and pollen, and the number of the characteristic peptides was only 61 and 25, respectively. Analyses of esterases and acid phosphatases from young leaves and pollen led to identification of isoenzymes characteristic of the three cultivars. Only for Frankasol no specific acid phosphatase was observed, both in leaves and in pollen.

3

Leukocyte acid phosphatase and selected haematological indices in BLV infected cows

75%

Kaczmarczyk E., Czarnik U., Bojaroj B., Walawski K.

Journal of Applied Genetics

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1999

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vol. 40

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issue 2

93-101

EN

Acid phosphatase of blood leukocytes (AcP) is a lysosomal enzyme which occurs in granulocytes and lymphocytes, but is not found in monocytes. In cattle, the occurrence of AcP polymorphism appeared in the form of A and AB phenotypes controlled by two autosomal alleles. A statistically highly significant repeatability was observed for AcP activity measured in lymphocytes from cows resistant to BLV (bovine leukaemia virus) infection. The highly inherited AcP activity and monogenic nature of AcP polymorphism in cattle allowed us to find out an association between AcP polymorphism and activity of AcP as well as haematological indices. In this study, 60 cows reared in one herd were analysed. The blood samples were collected in the last month before calving and in the first week after calving. The results obtained from cows with phenotype A revealed a statistically higher activity of AcP in lymphocytes whereas a lower activity of this enzyme was recorded in granulocytes. Furthermore, statistically significant differences were also observed in leukocyte number, percentage of lymphocytes and percentage of neutrophils.

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3 Journal of Applied Genetics

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1 Fiedorowicz A.

1 He L.

1 Kalinowski A.

1 Klimko M.

1 Kluza M.

1 Walawski K.

Leukocyte acid phosphatase and metabolic efficiency of phagocytes in the first lactation trimester of cows from a leukaemic herd

Two-dimensional electrophoresis patterns of total proteins, esterases and acid phosphatases from pollen, seeds and leaves of three cultivars of Helianthus annuus L.

Leukocyte acid phosphatase and selected haematological indices in BLV infected cows