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1

Physiological evaluation of in vitro cultivated plants

100%
Borkowska B.
Biotechnologia
|
2001
|
issue 2
133-136
EN
The aim of the present work was to evaluate the morphological and physiological status of strawberry shoots (cv. Senga Sengana) cultivated in vitro and their subsequent (out of glass vessels) ability to form plantlets with developed autothrophic metabolism. Standard medium recommended by Boxus was supplemented with glucose or sucrose 30 g/l. Biomass production and particular shoot formation were more efficient in the presence of glucose. The capacity of the shoots to form the root system and to develop photosynthetic activity was higher for shoots taken from the glucose-medium than the sucrose containing medium.
2

Rooting in vitro and acclimatization in the greenhouse of herbaceous peony plantlets

88%
Gabryszewska E., Kawa-Miszczak L.
Biotechnologia
|
2010
|
issue 2
172-179
EN
The influence of sucrose concentration (10 and 30 g l-1), low level of nitrogen salts (1/8 KNO3, 1/8 NH3NO4), auxins (IBA 1 mg l-1 + IAA 1 mg l-1 + NAA 0,01 mg l-1), and temperature (15?C, 20?C) on the rooting in vitro and acclimatization in the greenhouse of two cultivars ('Jadwiga', 'Professor Wojcicki') of Paeonia lactiflora were investigated. The level of endogenous carbohydrates in the peony shoots and roots during the rooting and acclimatization phases were analysed. There were a higher rooting and acclimatization percentage of 'Professor Wojcicki' cultivar than 'Jadwiga' cultivar . Also, more roots were produced by shoots of 'Professor Wojcicki' cultivar . The presence of auxins in the medium and the higher concentration of sucrose (30 g l-1) stimulated number of roots/shoot. On the other hand, the higher rooting percentage was found on the auxin?free medium in the presence of high level of sucrose. The shoots of 'Professor Wojcicki' rooted best when cultured at 20?C, but the shoots of 'Jadwiga' at lower temperature - 15?C. During the rooting stage, the major sugars detected in the peony microplants were sucrose, glucose and fructose. After four weeks of acclimatization, the plantlets accumulated starch and showed strong inhibition of shoot growth, and dormant buds were developed.
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