Search results

Search:
in the keywords: 87.85.Pq

Sort By:

Limit search:

Small Animal PET as Non-Invasive Tool for Preclinical Imaging

100%

Oteo M., Morcillo M.

Acta Physica Polonica A

2015

vol. 127

issue 5

1483-1486

Increasingly, in vivo imaging of small laboratory animals has become critical for preclinical biomedical research allowing the non-invasive assessment of biological and biochemical processes in living subjects. Many different positron labeled compounds have been synthesized as tracer that target a range of specific markers or pathways yielding quantitative, spatially and temporally indexed information on normal and diseased tissues such as tumors. Because of its non-invasive nature, nuclear techniques also enable to repeat studies in a single subject, facilitating longitudinal assay designs of rodent models of human cancer, cardiovascular, neurological and other diseases over the entire process, from the inception to progression of the disease, and monitoring the effectiveness of treatment or other interventions (with each animal serving as its own control and thereby reducing biological variability). This also serves to reduce the number of experimental animals required for a particular study. In contrast to cell or tissue culture-based experiments, studies in intact animals incorporate all the interacting physiological factors present in vivo.

Methodology for FTIR Imaging of Individual Cells

100%

Yao S., Cestelli Guidi M., Delugin M., Della-Ventura G., Marcelli A., Petibois C.

Acta Physica Polonica A

2016

vol. 129

issue 2

250-254

FTIR imaging is a novel spectroscopic technique able to provide cell imaging, in vivo and in real-time. However, one key issue is developing methodologies for cell culture on IR-transparent substrates fitting cell biology requirements. In this work we tested different IR-transparent substrates in terms of biotoxicity, surface properties, and spectral image acquisition qualities. Only a few substrates, namely Si₃N₄, Ge, GLS, LaF₃, Si, SrF₂, ZnS/C, ZnS/F, were found to provide cell culture conditions comparable to those observed on usual polycarbonate Petri dishes, the main limiting parameter being the toxicity of the material (ZnS, GLS, PbF₂, PbCl₂) or a poor adhesiveness (notably diamond, AgCl, CaF₂, ZnS). From substrates eligible for a good-quality cell culture, the spectral acquisition quality is mainly affected by the refractive index value. Finally, the best compromise between cell culture quality and image spectral quality could be obtained using Si and Ge substrates. This rationalization of the available IR-transparent substrates for bioimaging is particularly relevant for live cell analyses, where cell culture conditions must remain unaffected by substrate properties.

Refine search results

2 Acta Physica Polonica A

1 2016

1 2015

1 Cestelli Guidi M.

1 Della-Ventura G.

1 Delugin M.

1 Marcelli A.

1 Morcillo M.

1 Oteo M.

1 Petibois C.

1 Yao S.

Search results

Small Animal PET as Non-Invasive Tool for Preclinical Imaging

Methodology for FTIR Imaging of Individual Cells