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New Possibilities Opened by Synchrotron Spectromicroscopy in Neurobiology

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De Stasio G.

Acta Physica Polonica A

1997

vol. 91

issue 4

715-721

The use of synchrotron spectromicroscopy in neurobiology is definitely beyond the feasibility-test stage. It produced a large variety of results, mostly concerning the microscopic localization of transition elements in neural cells. We will first describe the experimental technique, then we will discuss some specific experimental examples, including the uptake of cobalt and zinc in rat cerebellar neurons.

Time-Resolved X-Ray Diffraction Studies on Biological Systems

100%

Rapp G.

Acta Physica Polonica A

1992

vol. 82

issue 1

103-120

Understanding of biological function requires knowledge both on structural and dynamical aspects. The aim of time-resolved X-ray diffraction is to combine structural and kinetic methods by monitoring structural or conformational changes within a single protein or assemblies of proteins during their biological action in real time. This requires (i) a powerful X-ray source, (ii) appropriate detectors capable of dealing with high local and total count rates and (iii) a suitable trigger mechanism. These aspects are briefly discussed with emphasis on light as trigger for initiation of structural or conformational changes. Examples are presented on muscle contraction, lipid phase transitions, the photocycle of bacteriorhodopsin and the application of Laue crystallography on the protein p21.

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2 Acta Physica Polonica A

1 1997

1 1992

1 De Stasio G.

1 Rapp G.

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New Possibilities Opened by Synchrotron Spectromicroscopy in Neurobiology

Time-Resolved X-Ray Diffraction Studies on Biological Systems