The electron spin resonance of γ-irradiated single crystals of acetylcholine β-resorcylate has been observed and analyzed for different orientations of the crystals in a magnetic field. C_{14}H_{21}NO_{6} acetylcholine β-resorcylate single crystals and powders have been investigated between 120 and 360 K. The spectra were found to be isotropic down to 120 K. The isotropic value of g-factor and the hyperfine splitting constant of protons were found to be 2.0030 and 1.85 mT, respectively. The measurements indicate that 80% of unpaired electron is localized on the carbon and 20% is on the oxygens. The results are consistent with the literature data of the CH_{3}COO¯ radical.
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