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1

Hydrolysis of Lactose from Cheese Whey Using a Reactor with β-Galactosidase Enzyme Immobilised on a Commercial UF Membrane

100%

Regenhardt S. A., Mammarella E. J., Rubiolo A. C.

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vol. 34

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issue 3

375-385

EN

In this study, β-galactosidase enzyme from Kluyveromyces fragilis was immobilised on a commercial polyethersulfone membrane surface, 10 kDa cut-off. An integrated process, concerning the simultaneous hydrolysis-ultrafiltration of whey lactose was studied and working conditions have been fixed at 55°C and pH 6.9, the same conditions that are used for the industrial process of protein concentration. For the immobilisation, best results were obtained using 5% (v/v) of glutaraldehyde solution and 0.03 M galactose; the total activity recovery coefficient (TARC) was 44.2%. The amount of immobilised enzyme was 12.49 mg with a total activity of 86.3 LAU at 37°C, using 5% (w/v) lactose solution in phosphate buffer (100 mM pH 6.9). The stability of the immobilised enzyme was approximately 585 fold higher in comparison with the stability of free enzyme. Multipoint covalent immobilisation improves the stability of the enzyme, thereby enhancing the decision to use the membrane as a filtering element and support for the enzyme immobilisation.

2

Low-glucose medium induces ORP150 expression and exerts inhibitory effect on apoptosis and senescence of human breast MCF7 cells

100%

Krętowski R., Stypułkowska A., Cechowska-Pasko M.

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2013

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vol. 60

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issue 2

167-173

EN

Glucose deprivation is a factor evoking endoplasmic reticulum (ER) stress and induction of expression of an oxygen-regulated protein of 150 kDa (ORP150). We studied the effect of inducible overexpression of ORP150 on senescence and apoptosis of human breast carcinoma cells (MCF7) and human skin fibroblasts. We found an inhibitory effect of ORP150 on apoptosis and senescence of MCF7 cells, but not fibroblasts in ER stress conditions. An increased expression of senescence-associated β-galactosidase and acid β-galactosidase activity (biomarkers of cellular senescence) was observed. We suggest that ORP150 induction in cancer cells can promote tumour progression and may be a major cause of their resistance to chemotherapeutics.

3

Hydrolysis of Lactose from Cheese Whey Using a Reactor with β-Galactosidase Enzyme Immobilised on a Commercial UF Membrane

88%

Regenhardt S. A., Mammarella E. J., Rubiolo A. C.

Chemical and Process Engineering

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2013

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issue 3

4

Determination of lysosomal exoglycosidases in human saliva

75%

Chojnowska S., Zalewska A., Knaś M., Waszkiewicz N., Waszkiel D., Kossakowska A., Zwierz K.

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2014

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vol. 61

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issue 1

85-90

EN

Background: Currently we observe a growing interest in human saliva as a non-invasive material for diagnosis and monitoring of general and oral diseases. Methods: The aim of our study was adaptation of the Marciniak et al. (Marciniak J, Zalewska A, Popko J, Zwierz K, 2006, Clin Chem Lab Med 44: 933-937) method for determination of HEX and GLU activity in synovial fluid, and for determination of: HEX and GLU, as well as MAN, GAL, and FUC activity in human saliva. Results: Under optimal conditions, 10 μl of saliva for HEX, and 30 μl for GLU, MAN, GAL and FUC, were sufficient for determination of human salivary exoglycosidases activity with variation coefficient ranging from 0.89 for GLU to 0.99 for GAL. Conclusion: The adapted method for exoglycosidases activity determination in human saliva is sufficiently sensitive and precise to use in clinical diagnosis.

5

Activity of lysosomal exoglycosidases in submandibular glands of rats intoxicated by cadmium at doses related to human chronic environmental and occupational exposures.

75%

Zalewska A., ska M. M. B., Marciniak J., Karaszewska K., Zwierz K., Moniuszko-Jakoniuk J.

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vol. 51

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issue 3

831-837

EN

Work in cadmium (Cd) smelter and smoking cigarettes damages teeth and oral mucosa which are protected by tissue and salivary glycoconjugates: glycoproteins, glycolipids, and proteoglycans. We worked out a rat model imitating human "environmental" and "occupational" exposure to cadmium using 5 mg Cd and 50 mg Cd/l in drinking water, respectively. In submandibulary glands of exposed to Cd rats, we found the time and dose dependent accumulation of Cd and simultanous decrease in activity of β-N-acetylhexosaminidase (HEX). In homogenates of submandibulary glands of control rats, β-N-acetylhexosaminidase showed the highest activity. The activities of α;-mannosidase and β-galactosidase were very low. None of these exoglycosidases were inhibited by Cd even at 44 mM concentration.

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3 Acta Biochimica Polonica

2 Chemical and Process Engineering

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3 2013

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2 Mammarella E. J.

2 Regenhardt S. A.

2 Rubiolo A. C.

2 Zalewska A.

2 Zwierz K.

1 Cechowska-Pasko M.

1 Chojnowska S.

1 Karaszewska K.

1 Knaś M.

1 Kossakowska A.

1 Krętowski R.

1 Marciniak J.

1 Moniuszko-Jakoniuk J.

1 Stypułkowska A.

1 Waszkiel D.

1 Waszkiewicz N.

1 ska M. M. B.

Hydrolysis of Lactose from Cheese Whey Using a Reactor with β-Galactosidase Enzyme Immobilised on a Commercial UF Membrane

Low-glucose medium induces ORP150 expression and exerts inhibitory effect on apoptosis and senescence of human breast MCF7 cells

Hydrolysis of Lactose from Cheese Whey Using a Reactor with β-Galactosidase Enzyme Immobilised on a Commercial UF Membrane

Determination of lysosomal exoglycosidases in human saliva

Activity of lysosomal exoglycosidases in submandibular glands of rats intoxicated by cadmium at doses related to human chronic environmental and occupational exposures.