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Rehydration of CTMA Modified DNA Powders Observed by NMR

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Harańczyk H., Kobierski J., Zalitacz D., Nowak P., Romanowicz A., Marzec M., Nizioł J., Hebda E., Pielichowski J.

Acta Physica Polonica A

2012

vol. 121

issue 2

485-490

The rehydration of salmon sperm deoxyribonucleic acid (DNA) and cetyltrimethylammonium chloride (C_{19}H_{42}ClN) complexes was observed using hydration kinetics, sorption isotherm, and high power proton relaxometry (at 30 MHz). The hydration kinetics shows (i) a very tightly bound water not removed by incubation over silica gel (A_0^{h} = 0.061 ± 0.004), (ii) a tightly bound water saturating at A_1^{h} = 0.039 ± 0.011, with the hydration time t_1^{h} = (1.04 ± 0.21) h, a loosely bound water fraction (iii) with the hydration time t_2^{h} = (19.1 ± 3.2) h and the contribution progressively increasing with the air humidity. For the hydration at p/p_0 = 100%, after t_0 = (152.6 ± 2.5) h of incubation the swelling process begins. The swelling time was t_3^{h} = (12.5 ± 5.4) h, and the swelling amplitude A_3^{h} = 0.140 ± 0.016. The sorption isotherm is sigmoidal in form and is fitted by the Dent model with the mass of water saturating primary binding sites Δ M/m_0 = 0.102 ± 0.021. Proton free induction decay is a superposition of the immobilized proton signal (Gaussian, with T_{2S}* ≈ 30 μs) and two liquid signal components coming from tightly bound (T_{2 L_1}* ≈ 100 μs) and loosely bound water fraction with the amplitude proportional to the mass of water added (T_{2 L_2}* ≈ 1000 μs).

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1 Acta Physica Polonica A

1 2012

1 Harańczyk H.

1 Hebda E.

1 Kobierski J.

1 Marzec M.

1 Nizioł J.

1 Nowak P.

1 Pielichowski J.

1 Romanowicz A.

1 Zalitacz D.

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Rehydration of CTMA Modified DNA Powders Observed by NMR