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1

Manipulating the availibity of the ethylene receptors-consequences for in vivo and in vitro in plant development

100%
Kepczynski J.
Biotechnologia
|
2006
|
issue 4
36-48
EN
Plant hormone ethylene is involved in the regulation of many physiological processes. Response of plant tissue to ethylene requires binding of ethylene to its receptor, probably containing metal, perhaps cupper. Manipulation of ethylene perception allows to modulate physiological processes in order to know the role of ethylene and also to commercially control some processes. There are many compounds which interact with the ethylene receptor. Some compounds bind reversibly eg. 2,5-norbornadiene and some unreversibly eg. cyclopropenes bind to ethylene receptor. Cyclopropenes and 1-MCP are very effective blocking agents for ethylene receptor. Its application at low concentration, 0.5 nl, for 24 hours protects carnations and bananas from the effects of ethylene for ca. 12 days. 1-MCP is commonly used in studies and was registered to be used in a number of horticultural products in several countries. The effect of 1-MCP on in vivo seed germination, seedlings growth, flower senescence and fruit ripening as well as in vitro shoot formation, rhizogenesis and somatic embryogenesis are discussed.
2

Interference in ethylene biosynthetic pathway using transformation

63%
Kepczynski J., Kepczynska E.
Biotechnologia
|
2000
|
issue 2
72-82
EN
The biosynthesis of ethylene in plants and its regulation by manipulating the expression of ACC synthase or ACC oxidase genes are discussed. Ethylene synthesis can be reduced by the introduction of antisense ACC synthase or antisense ACC oxidase genes. Expression genes of SAM hydrolase from bacteriofage T3, which catalyze the conversion of SAM to methylothioadenosine, also diminished ACC availability. Another possibility of ethylene biosynthesis control is the expression of gene encoding ACC deaminase from Pseudomonas.
3

Plant growth regulators and somatic embryogenesis in Medicago sativa L.

63%
Kepczynski J., Kepczynska E.
|
EN
Somatic embryogenesis, resembling zygotic embryogenesis in vivo, is considered to be an efficient method of in vitro propagation of a number of agronomically important plant species including Medicago sativa L. and it offers an in vitro experimental system for studying the embryo development. Artificial seed technology is one of the important applications of the process. Induction of embryogenesis, embryo development and induction desiccation tolerance are affected by plant growth regulators. The review will focus on the effect of plant growth regulators: auxins, cytokinins, gibberellins, abscisic acid, jasmonates, ethylene and inhibitors on the synthesis and action of different phases of somatic embryogenesis in Medicago sativa L.
4

Ethylene and bacteria

51%
Kepczynska E., Zielinska S., Kepczynski J.
|
EN
The review article presents data on the ethylene emanation by bacteria, the two different pathways of its biosynthesis in these microorganisms and the role of ethylene in plant pathogenesis. ACC deaminase from Pseudomonas and Enterobacter spp., which catalyses the hydrolytic cleavage of ACC in higher plants was also discussed.
5

The effects of 2,4-D, NAA and picloram on callus induction and somatic embryogenesis in ornamental asparagus

51%
Kepczynski J., Zielinska S., Kepczynska E.
Biotechnologia
|
2001
|
issue 2
170-173
EN
In this study we examined the effect of 2,4-D, NAA and picloram at 1.5, 3.0 and 4.5 mg/l in MS medium on callus induction and somatic embryogenesis in Asparagus densiflorus cv. Sprengeri and Asparagus plumosus. The callus formation of both shoot tips and nodal explants from A. densiflorus after 8 weeks of culture on MS medium supplemented with NAA and picloram (1.5, 3.0 mg/l) was observed. However, callus on medium containing 2,4-D was occasionally induced. On medium supplemented with picloram shoot tip explants of Asparagus plumosus showed callus inducing capacity. After four weeks of callus culture on hormone-free medium the globular embryos were achieved. On callus induced NAA organogenesis was observed.
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