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Exploring Anopheles gambiae Giles, 1902, (Family: Culicidae) Doublesex Gene Editing via CRISPR and Its Implications for CAR T-Cell Therapy and Vector Control in Nigeria

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Jibril O. S., Oluyinka A. I., Faysal K. M., Jerry A. N., Waya N. S., Shahul H.

World News of Natural Sciences

2026

vol. 64

1-10

Advances in CRISPR-based editing and chimeric antigen receptor (CAR) T-cell engineering have pushed modern biomedical research into practical, high-impact space. This study reports a workshop-based investigation that applied CRISPR guideRNA design and promoter analysis methods routinely used in CAR T-cell design to the doublesex (dsx) gene of Anopheles gambiae. Using Ensembl and NCBI resources, multiple sequence alignment across Anopheles species and motif searches in the upstream region of dsx were performed to identify conserved target windows and a putative upstream regulatory region. The intron – exon boundary targeted by contemporary gene-drive strategies (intron4–exon5) proved highly conserved across sampled species, supporting its suitability for CRISPR targeting. A candidate promoter motif architecture, including a predicted TATA box and initiator element within a conserved upstream window, is presented as a putative regulatory locus for further experimental validation. The paper situates these findings in two applied contexts: precision promoter selection for CAR expression in therapeutic cell engineering, and CRISPR-driven mosquito population control with potential to reduce malaria burden in Nigeria. Limitations and ethical considerations are discussed with emphasis on the need for local, experimental follow-up.

Effects of fungus extract Pestalotiopsis microspora (Speg.) Bat. & Peres, 1966, on the antioxidant system of mice infected with Plasmodium berghei Vincke & Lips, 1948

100%

Faysal K. M., Adam O. A., Jibril O. S., Esther O. B., Jerry N. A., Latifah A. A., Waya N. S.

World News of Natural Sciences

2026

vol. 64

45-54

The experiment is focused on evaluating the effect of Pestalotiopsis microspora on the antioxidant system of mice infected with Plasmodium berghei. Twenty-five male albino mice of average weight of 20kg were distributed randomly into 5 groups. Infected untreated (group 1), Infected untreated (group 2), Infected treated with chloroquine (group 3) Infected treated with 25 mg/kg fungus extract (group 4), Infected treated with 50 mg/kg fungus extract (group 5). All the groups were infected with Plasmodium berghei via the intra-peritoneal route. After seven days’ post infection, infected treated with chloroquine (group 3) Infected treated with 25 mg/kg fungus extract (group 4), Infected treated with 50 mg/kg fungus extract (group 5) were treated with chloroquine, 25 mg/kg and 50 mg/kg fungus extract respectively. The treatment lasted for 4 days. The results of this study revealed that, the effect of the fungus extract significantly increased the ALT, catalase, MDA, uric acid and XO in serum of infected treated group when compared with the decrease in the uninfected untreated group.

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2 World News of Natural Sciences

2 2026

2 Faysal K. M.

2 Jibril O. S.

2 Waya N. S.

1 Adam O. A.

1 Esther O. B.

1 Jerry A. N.

1 Jerry N. A.

1 Latifah A. A.

1 Oluyinka A. I.

1 Shahul H.

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Exploring Anopheles gambiae Giles, 1902, (Family: Culicidae) Doublesex Gene Editing via CRISPR and Its Implications for CAR T-Cell Therapy and Vector Control in Nigeria

Effects of fungus extract Pestalotiopsis microspora (Speg.) Bat. & Peres, 1966, on the antioxidant system of mice infected with Plasmodium berghei Vincke & Lips, 1948