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Cytotoxicity of PP(Arg)2- and Hp(Arg)2-mediated photodynamic therapy and early stage of apoptosis induction in prostate carcinoma in vitro

100%

Nowak-Stępniowska A., Wiktorska K., Małecki M., Romiszewska A., Padzik-Graczyk A.

Acta Biochimica Polonica

2011

vol. 58

issue 4

497-505

Porphyrin photosensitizers tend to localize in mitochondria. The depolarization of mitochondrial membrane is one of the early stages of apoptosis and Laser Scanning Fluorescence Microscopy allows to determine changes in transmembrane mitochondrial potential under influence of PDT depending on the kind of photosensitizer (PP(Arg)2, Hp(Arg)2), the energy dose (5, 10, 30 and 50 J/cm2) and time periods (24 and 48 hours after irradiation) in the LNCaP (lymphonodal metastasis of prostate carcinoma, the androgen dependent cell line). Cyototoxicity induced by PP(Arg)2- and Hp(Arg)2-based PDT depending on energy dose and time after irradiation in prostate carcinoma is determined with MTT. Generally, it was shown that lower energy doses induce greater changes in transmembrane mitochondrial potential. Hp(Arg)2-based PDT was more effective causing greater mitochondrial membrane depolarization and cell viability decrease in comparison to PP(Arg)2-mediated PDT (in the case of maximal nontoxic photosensitizer doses used).

Application of electrophoretic methods for detection of protein-porphyrin complexes.

86%

Kowalska A., Kwiek P., Miłosz E., Gondek G., Romiszewska A., Graczyk A., Podhajska A. J.

Acta Biochimica Polonica

2003

vol. 50

issue 4

1155-1163

Simple methods for detection and isolation of protein-porphyrin complexes were elaborated in our laboratory. They are based on the separation of protein-porphyrin complexes in native polyacrylamide gel and measurement of their fluorescence, with the use of two detection systems: the commercially available Gel DocTM 2000 system, and a system specially designed for the purpose of these investigations, concerning protein-porphyrin interactions. The fluorescent complexes can be electro-transferred from the gel onto PVDF membrane, eluted and analyzed in order to identify the protein interacting with porphyrins.

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2 Acta Biochimica Polonica

1 2011

1 2003

2 Romiszewska A.

1 Gondek G.

1 Graczyk A.

1 Kowalska A.

1 Kwiek P.

1 Małecki M.

1 Miłosz E.

1 Nowak-Stępniowska A.

1 Padzik-Graczyk A.

1 Podhajska A. J.

1 Wiktorska K.

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Cytotoxicity of PP(Arg)2- and Hp(Arg)2-mediated photodynamic therapy and early stage of apoptosis induction in prostate carcinoma in vitro

Application of electrophoretic methods for detection of protein-porphyrin complexes.