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Application of the ProteomeLab™ PF2D protein fractionation system in proteomic analysis for human genetic diseases

100%
Wang L., Zhong N.
Open Chemistry
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2012
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vol. 10
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issue 3
836-843
EN
Proteomic analysis has been widely used in elucidating the mechanism of diseases. As a classical proteomic approach, two-dimensional gel electrophoresis (2DGE) has been commonly applied in finding differentially expressed proteins through a first dimension of separation by the isoelectric point (pI) of proteins and a second dimension of separation according to the molecular weight (MW) of proteins. Compared to 2DGE, a recently developed commercial system from Beckman Coulter, the two-dimensional protein fractionation (PF2D), separates proteins according to the pI of proteins in the first dimension followed by a second dimension of separation according to the degree of protein hydrophobicity. As a liquid-based fractionation system, PF2D could facilitate the extraction and separation of broader protein categories and improve reproducibility and quantification as well as be less labor-intensive, which are usually identified as limitations of a gel-based 2DGE platform. This review evaluates the applications of the PF2D system and discusses the perspectives and advantages of PF2D in the investigation of cancer and genetic disorders and in protein mapping in human biological fluids and cell cultures. [...]
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Convection Rolls and Individual Particles Movements in Horizontally Vibrated Granular Particles System

71%
Rehman A., Wu P., Li L., Zhang S., Wang L.
Acta Physica Polonica A
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2016
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vol. 130
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issue 6
1336-1342
EN
Convection in horizontally vibrated granular systems is significant for scientists and engineers for their importance in the field of mining, geo-physics, and pharmaceutical etc. This research work studied three types of convection rolls, "Homogeneous convection roll", "lower-right diagonal convection roll" and "upper-right diagonal convection roll" which occurred in a square container filled with binary granular particles mixture of sized d=(4.0±0.2) mm and d=(8.0±0.2) mm. Container was vibrated horizontally with low frequencies f and low dimensionless acceleration Γ. Helical movement was observed along the walls perpendicular to direction of motion while straight-line movement along the walls horizontal to direction of motion. Helical and straight-line movements of particles along the walls are the part of convection rolls. A heap appeared due to vibration, which has dominant effect on the convection rolls. Heap position is function of frequency f and dimensionless accelerations Γ.
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