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  1. 1 Biotechnologia

  2. 1 Journal of Applied Genetics

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  1. 2 2001

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  1. 2 Prazak R.

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1

Salt tolerance of Triticum monococcum L., T. dicoccum (Schrank) Schubl., T. durum Desf. and T. aestivum L. seedlings

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Prazak R.
Journal of Applied Genetics
|
2001
|
vol. 42
|
issue 3
289-292
EN
Responses to salt stress of diploid wheat Triticum monococcum L., tetraploid wheats T. dicoccum (Schrank) Schubl. and T. durum Desf. cv. Grandur, and hexaploid wheats T. aestivum L. cvs. Begra and Gama were studied. Seeds were germinated on filter paper in Petri dishes, moistened in Hoagland medium containing 0 (control), 10, 20, 50, 100 and 200 mM NaCl. Index of tolerance to salt treatments was calculated on the basis of shoot length. Increase in salt concentration led to decrease in seed germination and inhibition of shoot and root elongation. Hexaploid and diploid wheats were more tolerant to salt stress than tetraploid wheats.
2

Micropropagation of Dendrobium kingianum Bidwill orchid

100%
Prazak R.
Biotechnologia
|
2001
|
issue 2
144-147
EN
For the development of aseptic culture, Dendrobium kingianum Bidwill orchid pseudobulbs (2.0-3.0 cm long) with one or two terminal leaves were surface sterilized with 0.1% mercuric chloride solution and transferred to initial MS medium, supplemented with auxin IAA at 0.5 mg dm-3 and cytokinin BA at 1.0 mg dm-3. New shoots, which developed after 4-6 weeks of culture at the base of pseudobulbs or at their upper nodes, were transferred to basal MS medium suplemented with an auxin (IAA or NAA) at 0.5 and 1.0 mg dm-3 and cytokinin Kinetin or 6-benzylaminopurine (BA) at 0.5, 1.0 and 2.0 mg dm-3 in different combinations. The responses of orchid cultures varied according to concentrations and type of growth regulators. From tested growth regulators, BA in concentration 2.0 mg dm-3 in combination with 0.5 mg dm-3 IAA was the most effective for shoot induction. At 0.5 mg dm3 both IAA and NAA strongly stimulated shoots multiplication in comparison to 1.0 mg dm-3. NAA-BA combinations positively influenced the shoot length. For roots induction, the NAA-Kinetin combinations were better than the remaining ones. Kinetin stimulated the increase of the root length.
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