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1

Plant viruses as vectors for foreign gene expression in plants

100%
Palucha A.
Biotechnologia
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2002
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issue 1
105-112
EN
Several studies have demonstrated the use of plant viruses as vehicles to introduce and express nonviral genes in plants. Many plant viruses multiply to intensely in plants, leading to concomitantly high levels of nonviral gene expression. Plant virus expression vector technology can be improved along several lines. Not all viruses are equally sensitive to sequence manipulations and may be less prone to insert instability. New vectors and new expression strategies are needed to expand the range of plants in which the systems can be used. To increase the carrying capacity or the ability to express more than one nonviral gene, new vectors should be developed.
2

Transformation of cucumber Cucumis sativus L.

51%
Szwacka M., Burza W., Palucha A., Malepszy S.
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|
issue 4
20-26
EN
Different transformation methods were investigated. However a lot of fertile plants were obtained only in the case of A. tumefaciens and thaumatin with kanamycin as a selective marker. Small differences in the plant morphology and development were observed in T0 generation. Most of the plants showed the presence of the transgene but the level of mRNA was different.
3

Genetically engineered potato resistant to potato leafroll virus infection

51%
Palucha A., Chrzanowska M., Zagorski W., Hulanicka D.
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|
issue 4
38-47
EN
Binary plasmids carrying different fragments of the coat protein gene of potato leafroll luteovirus were constructed for transformation of commercial potato cultivar Bzura. Several transgenic plant lines were obtained and characterised. The resistance of transgenic plants to the virus was tested by inoculation with aphids or grafting. Selected transformants expressing viral RNA were resistant to virus challenge by viruliferous aphids. Expression of the antisense RNA prevented virus infection even after grafting with scions from infected plants.
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