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Polymorphisms of the 5,10-methylenetetrahydrofolate and the methionine synthase reductase genes as independent risk factors for spina bifida

100%

Pietrzyk J. J., Bik-Multanowski M., Sanak M., Twardowska M.

We analyzed the role of the C677T polymorphism of the 5,10-methylenetetrahydrofolate and the A66G polymorphism of the methionine synthase reductase genes as risk factors for occurrence of spina bifida. The studied population included 106 mothers and 104 children from affected families, and a control group of 100 adults. We found statistically significant differences between the occurrence of the homozygosity in these polymorphisms in the groups of mothers and children with thoracolumbal defects (C677T polymorphism) and lumbosacral defects (A66G polymorphism). We postulate that these polymorphisms should be regarded as independent risk factors for spina bifida.

Bioconjugation of Silver Nanowires with Photosynthetic Light-Harvesting Complexes

100%

Olejnik M., Twardowska M., Zaleszczyk W., Mackowski S.

Acta Physica Polonica A

2012

vol. 122

issue 2

357-364

We demonstrate a way to conjugate a light-harvesting complex, peridinin-chlorophyll-protein, with silver nanowires using biotin-streptavidin linker. In the case of conjugated structure we observe slight increase of the fluorescence intensity of the chlorophyll emission followed by the gradual decrease of the intensity due to photobleaching. For a non-conjugated mixture of peridinin-chlorophyll-protein with silver nanowires only the photobleaching takes place. The results suggest a possible way to fabricate hybrid nanostructures comprising light-harvesting complexes and metallic nanoparticles for achieving the efficient plasmon-induced enhancement of absorption of the light-harvesting complexes.

Fluorescence imaging of hybrid nanostructures composed of natural photosynthetic complexes and reduced graphene oxide

88%

Twardowska M., Chomicki D., Kamińska I., Niedziółka-Jönsson J., Maćkowski S.

Nanospectroscopy

2014

vol. 1

issue 1

Herein, we describe the results of fluorescence microscopy imaging of peridinin-chlorophyll-protein (PCP) photosynthetic complex mixed with reduced graphene oxide (rGO). Upon incorporation of rGO the fluorescence image of PCP changes substantially from one characterized by uniform intensity towards a more complex pattern. The isolated bright spots feature up to ten times higher emission intensity compared to the fluorescence of PCP in the reference sample, where no rGO was added. The number of the bright spots increases with increasing rGO concentration. At the same time the fluorescence intensity away from the bright spots in the PCP/rGO hybrid system is quenched in comparison to the PCP – only reference.

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1 Acta Physica Polonica A

1 Journal of Applied Genetics

1 Nanospectroscopy

1 2015

1 2012

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3 Twardowska M.

1 Bik-Multanowski M.

1 Chomicki D.

1 Kamińska I.

1 Mackowski S.

1 Maćkowski S.

1 Niedziółka-Jönsson J.

1 Olejnik M.

1 Pietrzyk J. J.

1 Sanak M.

1 Zaleszczyk W.

Search results

Polymorphisms of the 5,10-methylenetetrahydrofolate and the methionine synthase reductase genes as independent risk factors for spina bifida

Bioconjugation of Silver Nanowires with Photosynthetic Light-Harvesting Complexes

Fluorescence imaging of hybrid nanostructures composed of natural photosynthetic complexes and reduced graphene oxide