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Newly identified transcripts of UL4 and UL5 genes of human cytomegalovirus

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Gao S., Ruan S., Ma Y., Li M., Wang L., Zheng B., Qi Y., Sun Z., Huang Y., Ruan Q.

Human cytomegalovirus (HCMV) UL4 and UL5 genes are two members of the RL11 gene family. In an earlier study, three UL4 transcripts of about 1.7, 1.5 and 1.4 kb were found in early and late classes after infection by the Towne strain by nuclease protection and primer extension analyses. In the present study, two UL4 transcripts (1.5 and 1.7 kb) were found by cDNA library screening, Northern blot, 3' and 5' RACE analyses to appear initially in the immediate early phase and one UL4 transcript (1.4 kb) in the late phase in a low-passage clinical isolate. Furthermore, two novel low-abundance UL5 transcripts with the same 3' terminus as the identified UL4 transcripts in the UL4-UL5 gene region were found in late class RNAs.

Biochemical characterization of a catalase from Vibrio vulnificus, a pathogen that causes gastroenteritis

100%

Pei J., Wang H., Wu L., Xia S., Xu C., Zheng B., Li T., Jiang Y.

Acta Biochimica Polonica

2017

vol. 64

issue 3

543-549

Vibrio vulnificus is a virulent human pathogen causing gastroenteritis and possibly life threatening septicemia in patients. Most V. vulnificus are catalase positive and can deactivate peroxides, thus allowing them to survive within the host. In the study presented here, a catalase from V. vulnificus (CAT-Vv) was purified to homogeneity after expression in Escherichia coli. The kinetics and function of CAT-Vv were examined. CAT-Vv catalyzed the reduction of H2O2 at an optimal pH of 7.5 and temperature of 35°C. The Vmax and Km values were 65.8±1.2 U/mg and 10.5±0.7 mM for H2O2, respectively. Mutational analysis suggests that amino acids involved in heme binding play a key role in the catalysis. Quantitative reverse transcription-PCR revealed that in V. vulnificus, transcription of CAT-Vv was upregulated by low salinity, heat, and oxidative stresses. This research gives new clues to help inhibit the growth of, and infection by V. vulnificus.

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2 Acta Biochimica Polonica

1 2017

1 2015

2 Zheng B.

1 Gao S.

1 Huang Y.

1 Jiang Y.

1 Li M.

1 Li T.

1 Ma Y.

1 Pei J.

1 Qi Y.

1 Ruan Q.

1 Ruan S.

1 Sun Z.

1 Wang H.

1 Wang L.

1 Wu L.

1 Xia S.

1 Xu C.

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Newly identified transcripts of UL4 and UL5 genes of human cytomegalovirus

Biochemical characterization of a catalase from Vibrio vulnificus, a pathogen that causes gastroenteritis