Search results

Sort By:

Limit search:

Congo red interaction with α-proteins

100%

Sereikaite J., Bumelis V.-A.

Acta Biochimica Polonica

2006

vol. 53

issue 1

87-92

The ability of Congo red to form complexes with α-proteins, human growth hormone and human interferon-α2b, was found by absorption difference spectroscopy. A human growth hormone-Congo red complex was isolated by gel-permeation chromatography, and its visible absorption spectrum was registered in comparison to free dye. The ability of Congo red to induce dimerization of human growth hormone was demonstrated using chemical cross-linking agents 1,3,5-triacryloyl-hexahydro-s-triazine and ethylene glycol bis(succinimidylsuccinate).

Interaction of serum albumin with vinyl sulfonate azo dye

100%

Sereikaite J., Bumelis V.-A.

Open Chemistry

2008

vol. 6

issue 4

509-512

The interaction between bovine serum albumin and the mono azo reactive dye Orange ZT has been investigated using absorption difference spectroscopy. The influence of pH and ionic strength of the solution on the stability of the dye-protein complex has been determined. At 25°C, the complex dissociation constants were equal to 24.0, 28.0, 7.0, 11.0, 17.6 and 46.0 μM at pH 7.0, 6.5, 6.0, 5.5, 5.0 and 4.3, respectively. In the presence of 0.1, 0.2, 0.3 M KCl, at pH 6.0 and 25°C, the complex dissociation constants were 8.8, 20.0, 18.0 μM, respectively. The protein-dye complex dissociation constants show that Orange ZT could be used as an affinity ligand for protein purification. [...]

Refine search results

1 Acta Biochimica Polonica

1 Open Chemistry

1 2008

1 2006

2 Bumelis V.-A.

2 Sereikaite J.

Search results

Congo red interaction with α-proteins

Interaction of serum albumin with vinyl sulfonate azo dye