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1

Transfer and transgene expression

100%
Szopa J., Wrobel M.
Biotechnologia
|
2001
|
issue 1
63-72
EN
The development of transgenic plants strongly depends on the stable introduction of foreign nucleic acid into the plant genome. Recently, various transformation methods have been developed and successfully used for the development of transgenic plants of agricultural importance. Various vectors have been prepared, however the binary vector based on agrobacterial T-DNA is most commonly used. Of vectorless gene transfer systems, particle bombardment seems to be most useful. In both approaches, stable integration of DNA is based on random hybridization. The transformation efficiency is measured by the level of transgene expression and it may be potentially improved by different modifications, including insertion of multiple copies of promoter into a particular gene, insertion of procaryotic enhancer, increasing of mRNA stability, insertion of SAR/MAR sequences at the ends of gene, etc. Very recently it has been found that the increase of histone synthesis enhances transgene expression possibly as a result of increased transformation efficiency. Transgenic plants production also depends on the efficiency of the plant regeneration system which is used. There is no universally applicable method for regeneration of different tissues from various sources, thus regeneration protocol should be modified appropriately for each tissue and species.
2

Cell cultures and transgenic plants in biotechnology

100%
Szopa J., Kostyn K.
Biotechnologia
|
2006
|
issue 4
7-17
EN
The applications of cell cultures and transgenic plants in several aspects of modern biotechnology are reviewed. The usefulness of cell cultures in biosynthetic pathway investigation, micropropagation, development of new varieties, genetic mapping and gene functioning analysis and industrial production of bioactive compounds are pointed out. The emphasis of this review is also laid on scientific aspects of transgenic plants. They are successfully used for promoters and other gene regulatory sequences study, investigation of plant primary and secondary metabolite pathways and for improving both the productivity and quality of crops. Since there is a risk to transgenic plant technology mainly because of antibiotic resistance used for transgenes selection, several methods have been worked out for release marker gene which are also briefly reviewed.
3

The analysis of 14-3-3 gene family

81%
Szopa J., Czuj T., Lukaszewicz M.
|
2003
|
issue 3
95-106
EN
The 14-3-3s constitute a family of highly homologous proteins, first discovered in brain tissue and now thought to be present in all eukaryotic cells. Recently, thirteen cDNAs in Arabidopsis, seven in human cells and six in potato plant were found, all encoding highly homologous 14-3-3 protein isoforms. While there is substantial progress in the identification of diverse partners of 14-3-3 in recent years, at least two important questions need to be answered. Is there any specificity within 14-3-3 isoforms in the binding of diverse partners? Does this binding affects plant metabolism or physiology in vivo? The significance of 14-3-3 protein in potato metabolism has been shown by the use of transgenic plants in which 14-3-3 protein has been either increased by the expression of a Cucurbita pepo cDNA or decreased by an antisense RNA method. It was thus proposed that 14-3-3 protein affect the carbohydrate metabolism in potato via the regulation of catecholamine synthesis. To answer the question on isoform specificity, the isoforms gene promoters were first analysed for specific domains content by the comparison with the known sequences accumulated in database. Then, the promoter characteristic was studied in transgenic plants transformed with reporter GUS gene under the control of the 14-3-3 promoter. The data obtained strongly suggest that the function of particular isoform at least partially derives from promoter specificity.
4

Identification of genetic modifications in selected plants and foods using the PCR method

81%
Szopa J., Rembacz K., Lukaszewicz M., Skala J.
|
EN
In this paper we present an attempt to identify genetic modification of plants via PCR. Plants and plant products commercially available in Poland were tested. The results show that some of them are genetically engineered, however they are not marked as GMO.
5
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The effect of Linola and W92/72 transgenic flax seeds on the rabbit caecal fermentation - in vitro study

52%
Miśta D., Króliczewska B., Zawadzki W., Pecka E., Steininger M., Hull S., Żuk M., Szopa J.
Polish Journal of Veterinary Sciences
|
2011
|
issue 4
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