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1

Stimulating the effect of biotic elicitors on the production of pharmacologically active secondary metabolites in plant in vitro cultures

100%
Szpitter A., Krolicka A.
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issue 4
82-108
EN
Plant secondary products are the substances of great importance in many spheres of human life. In recent years, many methods have been investigated in order to increase yield of these compounds, synthesized in plant in vitro cultures ? systems, which proved to be very useful and efficient for this purpose. Among these techniques, biotic elicitation, although not yet applied to a large scale production, proved to be a very efficient procedure on laboratory scale. One of the major aims of the studies on biotic elicitation of plants is to identify universal and effective, but at the same time the cheapest and simplest elicitors which could be used to increase secondary metabolites' production in plant in vitro cultures. This review focuses on different biotic elicitors of complex composition (e.g.: fungal culture filtrates and homogenates), as well as those with a known structure (e.g.: chitosan or ergosterol). The factors influencing the elicitation process as well as ways of improving efficiency of this method by combining it with other techniques, which can also increase plant tissue productivity, are also discussed.
2

Elicitation of secondary metabolites in vitro culture of Ammi visnaga

100%
Krolicka A., Lojkowska E.
Biotechnologia
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2000
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issue 4
112-117
EN
Ammi visnaga (Umbelliferae) are subtropical annual plants, which contain two groups of pharmaceutically important substances: furanochromones and piranocoumarins. In order to check the possibility of the production of secondary metabolites, in vitro cultures of callus and cell suspension were established. The study was concentrated on the induction of production of secondary metabolites by exposing callus and cell suspension cultures to abiotic elicitors: acetylsalicylic acid, jasmonic acid and suspension of silicon dioxide and biotic elicitors: autoclaved lysates of Enterobacter sakazaki, and scleroglucan. Thin layer chromatography of methanol extracts of cultures of A. visnaga did not indicate high induction of secondary metabolites production. Treatment of the callus cultures of A. visnaga with acetylsalicylic acid or jasmonic acid induce accumulation of furanochromone - visnagin and piranocoumarin ? samidin. Exposing the callus and cell suspension cultures to the suspension of silicon dioxide indicated an induction of accumulation of furanochromone - kelolglucoside. Further research will concentrate on quantitative determination of the level of accumulated compounds.
3

Formation and characterization of hairy roots

100%
Wasilewska A., Krolicka A.
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issue 4
173-188
EN
Agrobacterium rhizogenes is a soil phytopathogen, which infects wounded plant tissue and generates overproliferation of neoplastic roots. Hairy-root cultures obtained by transformation of plant tissue by A. rhizogenes have evolved as an important tool for biosynthesis of plant secondary metabolites. This review discusses the methods for efficient plant transformation and hairy root formation for secondary metabolites production.
4

Resistance to antibiotics of the selected species of Agrobacterium rhizogenes

81%
Krolicka A., Kurlenda J., Lojkowska E.
Biotechnologia
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1999
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issue 2
94-103
EN
Agrobacterium rhizogenes is used for the transformation of plant cells and production of hairy roots cultures. In the presented work the bacteriostatic activity of several antibiotics on A. rhizogenes strains was tested. Different concentration of antibiotics belonging to the types of cefalosporin II and III generation, b-lactam and fluorochinolon were tested for elimination of the bacteria from transformed tissue. Out of all tested combinations, the mixture of carbenicilin and cefotaksym (claforan) was the most efficient for A. rhizogenes strains elimination from transformed plant tissues. The addition of those antibiotics to the regeneration medium was not toxic to plant tissues and it facilitated rapid growth of hairy roots.
5

Hairy root culture of Centaurium erythraea Rafn.

71%
Piatczak E., Wielanek M., Krolicka A., Lojkowska E., Wysokinska H.
Biotechnologia
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2004
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issue 2
29-36
EN
In this study, the transformed root culture of Centaurium erythraea was established, as a result of infection of leaves culture with agropine strain of Agrobacterium rhizogenes (LBA 9402). Frequency of root formation depended on the kind of explants and the presence of acetosyringone in the bacteria medium. The transformation was confirmed according to the analysis of opines and RAPD-PCR. As the result of transformation 30 clones of hairy roots were obtained. These clones have grown in liquid Woody Plant (WPM) medium without growth regulators. The curve of growth indicates that fresh weight of roots increased almost 17 times , and dry weight increased 12 times during 55 days. Hairy roots of C. erythraea were able to produce secoiridoid glycosides (sweroside, gentiopicroside, swertiamarine).
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