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1
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Reproductive Performance of Rabbit does Artificially Inseminated with Semen Supplemented with GnRH Analogue [des-Gly10, D-Ala6]-LH-RH Ethylamide

100%
Gogol P.
Polish Journal of Veterinary Sciences
|
2016
|
issue 3
2

Ferrous ion induced photon emission as a method to quantify oxidative stress in stored boar spermatozoa

63%
Gogol P., Pieszka M.
Folia Biologica
|
2008
|
vol. 56
|
issue 3-4
173-177
EN
The aim of the study was to evaluate the effect of semen storage on ferrous ion induced luminescence of boar spermatozoa and to determine the relationship between parameters of this luminescence and lipid peroxidation as measured by malondialdehyde (MDA) contents. Boar semen samples were diluted in Biosolwens extender and stored for 12 days at 15oC. Luminescence andMDA were measured directly after dilution (day 0) and at 6 and 12 days of semen storage. Luminescence was measured at 20oC using a luminometer equipped with a cooled photomultiplier with a spectral response range from 370 to 620 nm. Emission was induced by adding FeSO4 solution (final concentration 0.05mM). MDA content was measured by the HPLC method. The day of storage had a significant effect on some luminescence parameters and MDA content in spermatozoa. A significant correlation was observed between luminescence parameters andMDA concentration. The results of the study confirm that induced luminescence is strictly related to lipid peroxidation in spermatozoa that occur during boar semen storage. Parameters of luminescence treated as a holistic response of cells to oxidati semen preservation.
3
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Motility, mitochondrial membrane potential and ATP content of rabbit spermatozoa stored in extender supplemented with GnRH analogue [des-Gly10, D-Ala6]-LH-RH ethylamide

51%
Gogol P., Trzcińska M., Bryła M.
Polish Journal of Veterinary Sciences
|
2014
|
issue 4
4

Transvaginal method of bovine oocytes collection: aspiration efficiency, developmental competence of oocytes and application in breeding

51%
Smorag Z., Gogol P., Kuznetsov V.
Biotechnologia
|
1998
|
issue 2
153-160
EN
This paper describes the ultrasound-guided transvaginal method of oocyte collection in cattle. Both technical and physiological aspects affecting the efficiency of the method are presented. The results of the author's experiments on oocytes collection and their developmental competence is also discussed.
5
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Quality parameters and fertility of ram semen cryopreserved in egg yolk and soybean lecithin supplemented extenders

46%
Gogol P., Bryła M., Trzcińska M., Bochenek M., Gogol P., Bryła M., Trzcińska M., Bochenek M.
Polish Journal of Veterinary Sciences
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2019
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issue 1
6

The application of photon emission measurements for evaluation of semen biological value

39%
Godlewski M., Gogol P., Kwiecinska T., Laszczka A., Szczesniak-Fabianczyk B., Wierzuchowska D.
|
2003
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issue 1
116-228
EN
Improvements in the single photon counting method have made it possible to measure total emission intensity to monitor its kinetics and to make measurements (using cut-off filters) of the spectral distribution of electromagnetic radiation emitted from spermatozoa of three farm animals species. The association between peroxidation processes in cell membranes and the accompanying photon emission and sperm viability, sperm motility and metabolic processes was identified and further explored. The application part of the studies concerned the effect of stress factors on bull, ram and boar spermatozoa and the possibility of using ultraweak luminescence as a new biophysical method for evaluation of semen biological value. The obtained results indicate that measurements of ultraweak luminescence can prove useful for solving problems related to the evaluation, conservation and use of farm animal semen.
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