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1

Inhibition of RNA synthesis in vitro by 9-aminoacridine carboxamide antitumor agents. Effects on overall RNA synthesis and synthesis of the initiating dinucleotide

100%

Piestrzeniewicz M. K., Czyż M., Denny W. A., Gniazdowski M.

Acta Biochimica Polonica

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1990

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vol. 37

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issue 2

299-307

2

GATA-1 binding to the αV promoter negatively regulates expression of the integrin αV subunit in human leukemic K562 cells.

100%

Czyż M., Stasiak M., Boncela J., Cierniewski C. S.

Acta Biochimica Polonica

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2002

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vol. 49

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issue 1

19-28

EN

Recently we observed that the transcription factors Sp1 and Sp3 bind to the CTCCTCCTC sequence located between positions -194 and -172 of the αV promoter region and are directly involved in the regulation of transcriptional activity of the αV gene in human umbilical vascular endothelial cells (HUVECs) (Czyz & Cierniewski, 1999, Eur. J. Biochem. 265, 638). In this report we provide evidence that the GATA-1 factor regulates αV expression during differentiation of pluripotent K562 cells induced either by phorbol 12-myristate 13-acetate (PMA) or butyric acid (BA) through interaction with the GATA element in the αV gene promoter. DNase I footprinting analysis revealed that region -413 to -408, covering the GATA binding site, was protected by nuclear extract from K562 cells. There was no protection of this region by HUVEC nuclear extract. Electrophoretic mobility shift assay (EMSA) analysis of nuclear extract of K562 cells treated with BA revealed an increase in GATA binding activity, which was associated with reduced αV mRNA and αV protein on the cell surface. Stimulation of K562 cells with PMA resulted in opposite effects: lower expression of GATA-1 was associated with increased levels of αV. We conclude that the GATA-1 transcription factor specifically binds to the GATA element in the αV gene promoter and negatively regulates αV gene expression.

3

Actinomycin D specifically inhibits the interaction between transcription factor Sp1 and its binding site

99%

Czyż M., Gniazdowski M.

Acta Biochimica Polonica

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1998

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vol. 45

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issue 1

67-73

4

Covalent binding of nitroacrine (ledakrin, C-283) to nucleic acids and proteins

76%

Czyż M., Studzian K., Wasilewski A., Poziomska A., Wilmańska D., Gniazdowski M., Medical Academy of Łódź I. o. P. a. B.

Acta Universitatis Lodziensis. Folia Biochimica et Biophysica

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1988

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vol. 6

PL

W obecności związków sulfhydrylowych lek przeciwnowotworowy nitrakryna (Ledakrin, C-283) tworzy nieodwracalne kompleksy z DNA i RNA. Przedstawiono charakterystyką stabilności kompleksów w różnych warunkach.

5

Transcription factors as targets of anticancer drugs

62%

Gniazdowski M., Czyż M.

Acta Biochimica Polonica

|

1999

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vol. 46

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issue 2

255-262

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4 Acta Biochimica Polonica

1 Acta Universitatis Lodziensis. Folia Biochimica et Biophysica

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5 Czyż M.

4 Gniazdowski M.

1 Boncela J.

1 Cierniewski C. S.

1 Denny W. A.

1 Medical Academy of Łódź I. o. P. a. B.

1 Piestrzeniewicz M. K.

1 Poziomska A.

1 Stasiak M.

1 Studzian K.

1 Wasilewski A.

1 Wilmańska D.

Inhibition of RNA synthesis in vitro by 9-aminoacridine carboxamide antitumor agents. Effects on overall RNA synthesis and synthesis of the initiating dinucleotide

GATA-1 binding to the αV promoter negatively regulates expression of the integrin αV subunit in human leukemic K562 cells.

Actinomycin D specifically inhibits the interaction between transcription factor Sp1 and its binding site

Covalent binding of nitroacrine (ledakrin, C-283) to nucleic acids and proteins

Transcription factors as targets of anticancer drugs