Search results

1

Wide hybridization in the Triticeae involving Hordeum, Triricum, Triticale and Hordeum x Secale

100%

Wojciechowska B., Pudelska H.

Genetica Polonica

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1994

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vol. 35

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issue 4

221-230

EN

Intergeneric hybridization was carried out between various accessions of Hordeum jubatum (4x) and cultivars of Triticum aestivum (6x) and Triticale (6x) as well as T.monococcum (2x) and between cultivars of Triticum aestivum (6x) and the hybrid (Horodeum jubatum 4x X Secale cereale 4x) in both directions.The hybrid progeny was obtained via embryo culture from crosses of H.jubatum x T.aestivum and T.aestivum x(H.jubtum 4x X S.cereale 4x).The hybrid of H.jubatum x T.aestivum was produced at the frequency of 1.7% in relation to pollinated florets and five hybrids of T.aestivum x (H.jubatum 4x x S.cereale 4x) from successful combinations were produced at the frequency of 2.00-4.88%.The hybrids exibited varitionin somatic chromosome number.In meiocytes of two T.aestivum x (H.jubatum 4x X S.cereale 4x) plants a high chromosome instability was also found.The chromosomes were associated mostly as univalents, but some pairings (0.03-3.50 per cell) mainly as rod bivalents were observed.

2

Production and morphology of the hybrids Aegilops kotschyi ? Secale cereale and Ae. biuncialis ? S. cereale

100%

Wojciechowska B., Pudelska H.

Journal of Applied Genetics

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2002

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vol. 43

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issue 3

279-285

EN

Hybrids between Aegilops kotschyi and Ae. biuncialis with Secale cereale were synthesized. Five Ae. kotschyi and four Ae. biuncialis accessions, as well as one inbred and four self-compatible forms of Secale cereale were used for crossing. The hybrids were produced directly from cultured embryos or through embryo callus culture. Sixty hybrids, 11 involving Ae. kotschyi and 49 Ae. biuncialis, had a stable somatic chromosome number 2n = 3x = 21. The plants showed good vegetative vigour and tillering capacity. Morphologically the hybrids were intermediate between their parents and completely sterile. In vitro propagation of Ae. kotschyi and Ae. biuncialis ? S. cereale hybrids revealed that their capacity for callus production and plantlet regeneration ? varies.

3

Hybrids and amphiploids of Aegilops ovata L. with Secale cereale L.: production, morphology and fertility

100%

Wojciechowska B., Pudelska H.

Journal of Applied Genetics

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2002

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vol. 43

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issue 4

415-421

EN

Hybrids (2n = 3x = 21) between Aegilops ovata and Secale cereale were produced via embryo rescue. Most hybrid morphological traits were intremediate between parents, the plants grew vigorously but were completely sterile. The average frequency of chromosome associations at metaphase I was 19.57-20.19 univalents and 0.40-0.86 rod bivalents. The fertility of the hybrids was restored by doubling their chromosome numbers by colchicine treatment and in vitro propagation. Selfed seeds were obtained from colchicine-doubled sectors and some callus regenerates. The seeds were mostly well formed and developed vigorous plants without embryo culture. Colchicine- and callus-derived amphiploids (2n = 6x = 42) resembled the F1 plants in overall morphology, but showed a lower tillering ability, broader leaves, thicker culms and larger spikes.

4

Production and characterization of amphiploids of Aegilops kotschyi and Ae. biuncialis with Secale cereale, and of backcross hybrids of Ae. biuncialis ? S. cereale amphiploids with 2x and 4x S. cereale

100%

Wojciechowska B., Pudelska H.

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2005

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vol. 46

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issue 2

157-161

EN

Amphiploids (2n = 6x = 42) of Ae. kotschyi and Ae. biuncialis with self-compatible S. cereale were produced from F1 sterile hybrids (2n = 3x = 21) through colchicine treatment and callus tissue regeneration. The amphiploids resembled the F1 plants in overall morphology, but were larger in all respects and self-fertile. The spikelets consisted mostly of 3 well-developed florets. Selfed seeds were obtained from some colchicine-doubled sectors and callus regenerates. Most of the produced seeds were well developed. Backcrosses between amphiploids and rye (2x and 4x) resulted in obtaining (Ae. biuncialis ? S. cereale amphiploid) ? S. cereale hybrids via embryo culture. The BC1 plants (2n = 4x = 28 and 2n = 5x = 35, respectively) were phenotypically intermediate between the parents and vigorous in vegetative growth. Some seeds were obtained only from the 35-chromosome BC1 hybrids.

5

Embryo-callus regenerated hybrids and amphidiploids of barley cultivars x rye inbred lines

100%

Wojciechowska B., Pudelska H.

Journal of Applied Genetics

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1995

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vol. 36

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issue 4

313-323

EN

In the presented hybridization programme of barley cultivars and rye inbred lines including 48 cross combinations the seed set ranged from 3.13 to 92.98%, while embryos were formed in 0.74 to 36.36% in successful pollinations.Sixty five plants were generated by embryo callus culture and one - by embryo culture without callus formation.The hybrids had somatic chromosome numbers 2n=14 (60 plants) and 2n=28 (6 plants).Plants obtained vie embryo callus culture showed good vegetative vigour and well-developed root system.Spike morphology of all plants resambled that of rye.Meiosis in 17 diploids showed 0.13-0.63 barley-barley and rye-rye bivalents with a chiasma frequency of 0.14-0.69 per cell. The hetromorphic bivalent-like configurations occured in five plants in 0.01-0.02 per cell.The amphidiploids had 7.79-10.71 barley-barley and rye-rye bivalents with the chiasm frequency of 9.36-17.75 per cell.All plants, with 14 and 28 chromosomes, were completly sterile both in backcrosses and when selfed.

6

Impact of milk yield on pharmacokinetics of six intramammary drugs – a population approach

81%

Grabowski T., Burmańczuk A., Wojciechowska B., Kowalski C.

Polish Journal of Veterinary Sciences

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2018

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vol. 21

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issue 2

7

Rapid assessment of early embryo development in some wide crosses of cereals

81%

Slusarkiewicz-Jarzina A., Ponitka A., Wojciechowska B., Wozna J.

Journal of Applied Genetics

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2000

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vol. 41

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issue 2

75-80

EN

The cleared-ovule technique was evaluated for rapid examination of early embryo development in selfed (barley, wheat) and crossed (barley x rye and reciprocal barley x wheat) material. The pistils were fixed in FAA and the isolated ovules were cleared in methyl salicylate. The development of embryos and endosperm were observed at 24, 48, 72 and 96 hours after pollination. The embryo development rate in barley x rye crosses was mostly similar but in some cases delayed or slightly faster than in control mother cultivars. In contrast to barley x rye crosses, in the reciprocal barley x wheat crosses the frequency of embryos was lower. The study shows that the technique of cleared-ovule in methyl salicylate may be applied for rapid assessment of early embryo and endosperm development in cereal plants. In comparison with traditional sectioning methods the clear ovule technique is simpler, more efficient and quicker.

8

Isozymes in Aegilops kotschyi and Ae. biuncialis ? Secale cereale hybrids and Ae. kotschyi ? S. cereale amphiploids in relation to their parents

81%

Kalinowski A., Wojciechowska B., Borzyszkowska E.

Journal of Applied Genetics

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2003

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vol. 44

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issue 1

35-43

EN

Seven enzymatic systems in F1 Aegilops kotschyi and Ae. biuncialis ? Secale cereale hybrids, Aegilops kotschyi ? S. cereale amphiploids and their parental species (Ae. kotschyi, Ae. biuncialis and S. cereale) were analysed by starch and polyacrylamide gel electrophoresis. Five of them (phosphoglucose isomerase, glutamic oxalacetic transaminase, esterase, acid phosphatase, and diaphorase) were polymorphic and two (malic dehydrogenase and superoxide dismutase) were monomorphic. Several isophorms of phosphoclucose isomerase, esterase, acid phosphatase, and diaphorase were detected in some hybrids and amphiploids, but absent in the parents. The role of regulators, translocations and recombination is discussed in relation to the origin of these new isophorms. Some parental isozymes were absent both in hybrids and amphiploids, probably as a result of the suppression of structural genes in new combinations of the three genomes.

9

Aegilops-rye amphiploids and substitution rye used for introgression of genetic material into rye (Secale cereale L.)

81%

Apolinarska B., Wisniewska H., Wojciechowska B.

Journal of Applied Genetics

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2010

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vol. 51

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issue 4

413-420

EN

The valuable genes of Aegilops biuncialis, Ae. ovata, Ae. kotschyi, and Ae. variabilis were transferred to rye, by crossing Aegilops-rye amphiploids with tetraploid and diploid substitution rye. The C-banded karyotype of the BC1 and BC2 generations of amphiploids with 4x substitution rye and BC1 with 2x substitution rye showed great variation in chromosome number and composition. In the BC1 generation of amphiploids with 4x and 2x substitution rye, seed set success rate and germination rate varied depending on origin. However, plant sterility in all cross combinations of amphiploids with 4x and 2x substitution rye resulted in their elimination from further experiments in the BC3 and BC2 generations, respectively. In backcrosses of 4x substitution rye with amphiploids Ae. variabilis x rye 4x, fertile 4x rye plants containing Aegilops chromatin were produced in the BC2 generation.

10

Validation of relationship between milk resistance and daily yield of dairy cows

71%

Wojciechowska B., Burmańczuk A., Grabowski T., Kowalski C., Hola P.

Polish Journal of Veterinary Sciences

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2017

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issue 4

11

Screening for germline p53 mutations in pediatric and adult patients of high-risk groups in Poland

52%

Fiszer-Maliszewska L., Czernik J., Sawicz-Birkowska K., Perek D., Kozera M., Wojciechowska B., Kazanowska B., Hudziec P., Kilar E.

Archivum Immunologiae et Therapiae Experimentalis

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2000

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vol. 48

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issue 4

309-315

EN

Germline mutations of the p53 gene lead to cell transformation in various tissues. Such a complex cancer phenotype makes it difficult to recognize the carriers of the defective allele. Several studies undertaken to identify high-risk groups found germline p53 mutations in familial cancer aggregations and in patients with multiple tumors. We screened 189 pediatric and 48 adult patients. The high-risk groups comprised 41 patients with a family history of cancer and 35 with multiple neoplasms. Furthermore, 124 tumors were screened for somatic mutations. p53 exons 2 to 11 were analyzed by polymerase chain reaction and single strand conformation polymorphism (PCR-SSCP) followed by direct sequencing of abnormal DNA fragments. No germline p53 mutations were found and somatic mutations were detected in 5 of 59 sarcomas, globally, in 8 of 124 tumors. In conclusion, in Poland, p53 alterations do not seem very important for the predisposition to malignancy and development of sarcomas.

12

Family with Li-Fraumeni syndrome and no evidence of a germline mutation of the p53 gene or chromosomal aberrations

38%

Sikorska A., Traczyk T. Z., Traczyk Z., Konopka K. L., Konopka L., Fiszer-Maliszewska F.-M. L., Fiszer-Maliszewska L., Wojciechowska W. B., Wojciechowska B., Pienkowska-Grela P.-G. B., Pienkowska-Grela B., Rygier R. J., Rygier J., Woroniecka W. R., Woroniecka R., Witkowska W. A., Witkowska A., Rusin R. M., Rusin M.

Journal of Applied Genetics

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2001

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vol. 42

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issue 3

379-384

EN

Li-Fraumeni syndrome is a rare autosomal, dominant trait of diverse types of cancers in children and young adults, with a predominance of soft tissue sarcomas, osteosarcomas, brain tumours, adrenocortical and breast carcinomas, as well as leukaemias. We present a family with an unusual cancer history fulfilling the criteria of Li-Fraumeni syndrome. Mutational analysis of the p53 gene in constitutional DNA of several affected members of the family did not show any germline p53 defect. Cytogenetic studies did not reveal any structural aberrations.

Refine search results

Wide hybridization in the Triticeae involving Hordeum, Triricum, Triticale and Hordeum x Secale

Production and morphology of the hybrids Aegilops kotschyi ? Secale cereale and Ae. biuncialis ? S. cereale

Hybrids and amphiploids of Aegilops ovata L. with Secale cereale L.: production, morphology and fertility

Production and characterization of amphiploids of Aegilops kotschyi and Ae. biuncialis with Secale cereale, and of backcross hybrids of Ae. biuncialis ? S. cereale amphiploids with 2x and 4x S. cereale

Embryo-callus regenerated hybrids and amphidiploids of barley cultivars x rye inbred lines

Impact of milk yield on pharmacokinetics of six intramammary drugs – a population approach

Rapid assessment of early embryo development in some wide crosses of cereals

Isozymes in Aegilops kotschyi and Ae. biuncialis ? Secale cereale hybrids and Ae. kotschyi ? S. cereale amphiploids in relation to their parents

Aegilops-rye amphiploids and substitution rye used for introgression of genetic material into rye (Secale cereale L.)

Validation of relationship between milk resistance and daily yield of dairy cows

Screening for germline p53 mutations in pediatric and adult patients of high-risk groups in Poland

Family with Li-Fraumeni syndrome and no evidence of a germline mutation of the p53 gene or chromosomal aberrations