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From DNA sequence to antibiotic

100%
Pawlik K., Kotowska M.
Biotechnologia
|
2005
|
issue 3
57-74
EN
Progress in molecular biology, large number of known bacterial sequences and a number of microbiological genome sequencing projects finished or running allow to think about creating in vitro the DNA code in order to obtain new metabolites. Here, we describe the attempts of biosynthesis of new polyketides by designing new genes coding polyketide synthases. In assembling new polyketide synthases, the fragments of known genes are widely used. High homology of the polyketide synthase genes and a number of known produced polyketides give a possibility to describe a polyketide synthase on a DNA sequence level. Basing on known amino acid motifs, it is possible to determine enzymatic activities acting in the process of polyketide synthesis, and so to predict putative structure of the final metabolite. Successful artificial synthesis of 6-deoxyerythronolide B synthase genes show how near is the realization of the idea of producing new chemical compounds by creating DNA information.
2

DNA speed reading course ? high-throughput DNA sequencing technologies

100%
Kotowska M., Zakrzewska-Czerwinska M.
Biotechnologia
|
2010
|
issue 4
24-38
EN
Development of high-throughput DNA sequencing technologies that omit time consuming and labour intensive cloning steps have opened unprecedented possibilities in life sciences. Massive scale generation of raw sequences requires constant improvement of computational methods of data analysis. New disciplines of genomics, metagenomics and transcriptomics have emerged which revolutionize experimental approach to different fields of biology. Both basic studies, such as species evolution or microbial ecology, and applied sciences of biotechnology and medicine greatly from the new tools available. In this article next-generation DNA sequencing technologies are reviewed. Information on data analysis and applications is also provided.
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