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Infraciliature and Cell Division of the Little Known Freshwater Ciliate Uroleptus cf. magnificus (Kahl, 1932) Olmo, 2000 (Hypotricha, Uroleptidae), and List of Published Names in Uroleptus Ehrenberg, 1831 and Paruroleptus Wenzel, 1953

100%

He W., Shao C., Shi X., Berger H.

Acta Protozoologica

2011

vol. 50

issue 3

In 1988, we found a large (250–400 × 80–150 μm in protargol preparations) Uroleptus-like hypotrich in a freshwater pond in Harbin, China. We studied the morphology of non-dividers and the cell division using protargol impregnation. Since we disregarded live observations and due to the lack of a modern revision of the uroleptids, a final identification was not possible. A detailed comparison with the most similar limnetic Uroleptus-like hypotrichs and with Rigidothrix goiseri revealed that the Chinese population is very likely identical with Uroleptus magnificus [basionym Holosticha (Paruroleptus) magnificus Kahl, 1932], a very rare species possibly confined to limnetic, stagnant water bodies of the holarctic region. Besides the large size, main features of U. cf. magnificus are: (i) about 80 adoral membranelles; (ii) three or four inconspicuous transverse cirri; (iii) 5–8 dorsomarginal kineties; (iv) the oral primordium originates de novo left of the postoral midventral cirri; (v) the frontal-ventral-transverse cirri anlagen of the proter and the opisthe originate via primary primordia; (vi) the left frontal cirrus of the proter originates from the middle portion of the disorganizing parental paroral; (vii) the parental endoral becomes the undulating membrane anlage for the proter; and (viii) the frontoterminal cirri originate in the plesiomorphic manner, that is, from the rearmost anlage. A compilation reveals that 59 species, subspecies, etc. have been described in or assigned to Uroleptus and Paruroleptus, but only about 50% of them seem to be true uroleptids. Many species of this predominantly limnetic group are little known.

In vitro co-stimulation of anti-tumor activity by soluble B7 molecules

88%

He W., Hu Z.-B., Liu F., Feng X.-Q., Zou P.

Acta Biochimica Polonica

2006

vol. 53

issue 4

807-813

In order to investigate the anti-tumor activity of a soluble B7-1/immunoglobulin G fusion protein and explore an effective method to eliminate immune escape of tumor cells, a recombinant vector encoding this fusion protein was constructed and constitutively expressed in Chinese hamster ovary cells. After purification with protein G affinity chromatography, the soluble fusion protein was tested for bioactivity. Results showed that the fusion protein could significantly increase the density of B7-1 molecules on WEHI-3 cells, a mouse leukemia cell line. Through allogeneic mixed lymphocyte tumor cultures, it was demonstrated that, with the presence of the first signal, it could also significantly enhance T cell activation and killing activity against WEHI-3 cells and interleukin-2 secretion by activated mouse T lymphocytes. The conclusion can be drawn that the soluble B7-IgG fusion protein has a potent capacity to generate or enhance anti-tumor immune response in vitro, and its clinical value deserves further investigation.

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1 Acta Biochimica Polonica

1 Acta Protozoologica

1 2011

1 2006

2 He W.

1 Berger H.

1 Feng X.-Q.

1 Hu Z.-B.

1 Liu F.

1 Shao C.

1 Shi X.

1 Zou P.

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Infraciliature and Cell Division of the Little Known Freshwater Ciliate Uroleptus cf. magnificus (Kahl, 1932) Olmo, 2000 (Hypotricha, Uroleptidae), and List of Published Names in Uroleptus Ehrenberg, 1831 and Paruroleptus Wenzel, 1953

In vitro co-stimulation of anti-tumor activity by soluble B7 molecules