Search results

Sort By:

Limit search:

Cloned cDNA of the pPAG genes (porcine Pregnancy-Associated Glycoprotein) as useful templates for proteomical and genomical diagnoses

100%

Panasiewicz G., Szafranska B.

Biotechnologia

2005

issue 1

47-60

The PAG (Pregnancy-Associated Glycoproteins) gene family and chorionic expressions of their products suggest an important role(s) of this glycoprotein family throughout pregnancy in various mammals (pig, cattle, sheep, goat, hourse, zebra, cat and mouse). Each cloned cDNA is a major source of template for recombinant PAG protein productions, necessitated for requirements at various diagnostic tests of early pregnancy in ruminants. Moreover, each cDNA may be applied as template for preparation of selective genetic tests (i.e. micro-arrays), can provide essential background for estimation of correlations between various PAG genotypes and physiological reproductivity of each animal. Aforementioned genetic tests presumably will allow for pre-selection of young indyviduals with effective reproductive performances.

Length polymorphism of PCR-amplified genomic fragments of the Pregnancy-Associated Glycoprotein (PAG) gene family in the pig and some other domestic and wild mammals

71%

Szafranska B., Panasiewicz P. G., Panasiewicz G., Waclawik W. A., Waclawik A.

Journal of Applied Genetics

2001

vol. 42

issue 3

335-349

Porcine pregnancy-associated glycoprotein genes (pPAG) are known as a multigene family, in which five members have been cloned and sequences of their cDNAs identified. Porcine PAG1 and pPAG3 genes, belonging to the pPAG1-like subfamily, both encode enzymatically inactive precursors. In contrast, cDNAs of pPAG2, pPAG4 and pPAG6 represent the pPAG2-like gene subfamily, encoding enzymatically active precursors. The objective of this study was to investigate the polymorphism of both pPAG-like gene subfamilies in the pig in comparison to other domestic species, including cattle, sheep and goat (Artiodactyla), their wild relatives (red deer and wild pig) and horse (Perissodactyla). This is the first paper indicating the polymorphism of the pPAG gene family, examined by lengths of amplified genomic fragments (PCR). Obtained PCR products were analysed in relation to five characterised cDNAs of pPAGs (pPAG1-like and/or pPAG2-like subfamilies) and according to one recognised structural exon-intron organisation of the pPAG2 gene, among at least eight pPAG2-like genes expected in the porcine genome. The highest polymorphism frequency of both pPAG1- and pPAG2-like gene subfamilies was found in the second region, exons 5 and 6 (with intron E). The length of PCR-amplified genomic fragments was approximately: 1043, 700, 600 and 193 bp. A high polymorphism frequency was found in the 3?-terminal fragment, corresponding to exons 7-9 (with introns G and H), more frequent the pPAG2-like gene subfamily. The length of PCR-amplified genomic fragments was approximately: 733, 650 and 356 bp. In contrast, PAG polymorphism was not detected in another region, encompassing exons 2-4 (with introns B and C). The length of PCR-amplified genomic fragments was approximately 279 bp in all examined genomes. In conclusion, amplification of various regions of the PAG gene family presents a relatively inexpensive PCR method of animal pre-selection with different genotypes. Such a pre-selection of animals is helpful for further gene number inquiry of the PAG gene family in each animal, then in related generations. The obtained results provide a useful background for a genetic marker preparation (by Southern analysis of the PAG family) that will presumably enable an economical early selection of young animals for effective reproduction.

Refine search results

1 Biotechnologia

1 Journal of Applied Genetics

1 2005

1 2001

2 Panasiewicz G.

2 Szafranska B.

1 Panasiewicz P. G.

1 Waclawik A.

1 Waclawik W. A.

Search results

Cloned cDNA of the pPAG genes (porcine Pregnancy-Associated Glycoprotein) as useful templates for proteomical and genomical diagnoses

Length polymorphism of PCR-amplified genomic fragments of the Pregnancy-Associated Glycoprotein (PAG) gene family in the pig and some other domestic and wild mammals