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EN
The effect of removal of K+ accmulation on K+ current in insect axonal membrane was observed. Experiments were performed on isolated giant axon of a cockroach using double oil gap technique. K+ accumulation was reduced by: (1) an outward water flow induced by non-electorlytes (urea, glucose) added to extracellular saline and (2) by an increase of non-specific permeability of axonal glial layer obtained after the application of DMSO. The conclusions are: (1) osmolar effect depends on the type of molecule used for osmotic shock, (2) increase of outward K+ current in conditions of high extraaxonal osmotic pressure is attributed to the decrease of K+ accumulation and outward water flow, (3) removal of K+ accumulation doesn't affect the kinetics and the time course of K+ current, (4) experiments confirmed the presence of an inactivating component in the axonal outward K+ current, (5) DMSO must be used cautiously as a solvent in electophysiological experiments.
EN
Natural neurotoxins are promising molecules in the actual search for the development of alternative pest management since chemical insecticides pose unacceptable risks to the environment and to health. The aim of the article is to describe the application of two electrophysiological methods (double-oil-gap technique used on cockroach isolated giant axon and microelectrode technique used on cockroach neurosecretory DUM cells in situ) to study neurotoxin effects on insect nervous system function.
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