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1

Identification of blood group A and B antigens in human glycophorin

100%
Podbielska M., Krotkiewski H.
Archivum Immunologiae et Therapiae Experimentalis
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2000
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vol. 48
|
issue 3
211-221
EN
Glycophorin A (GPA), the major sialoglycoprotein of human erythrocyte membranes, was isolated separately from blood group A and B erythrocytes using phenol-water extraction. After purification, performed as gel filtration in the presence of SDS, two glycophorin samples GPA-A and GPA-B were run, in duplicate, in SDS-PAGE and electroblotted onto Immobilon P. After staining with 1) anti-glycophorin antibody and 2) with relevant anti-blood group (A or B) antibody it was shown that the band pattern of the samples in each duplicate was the same. GPA-A and GPA-B samples were also degraded using Carlson degradation (-elimination in mild alkaline/strong reducing conditions) and from reaction products the fractions of O-glycans and N-glycans were isolated; they were used in hemagglutination inhibition test. It was shown that both sugar fractions derived from GPA-A did inhibit agglutination of blood group A erythrocytes by anti-A antibody, whereas oligosaccharide fractions derived from GPA-B inhibited agglutination of blood group B erythrocytes by anti-B antibody. These results, obtained using immunochemical methods, confirm the presence of blood group A and B determinants in the carbohydrate moiety of human glycophorin, derived from the blood group A or B erythrocytes, respectively.
2

The ABO blood group system - past and present

100%
Podbielska M., Krotkiewski H.
Postępy Higieny i Medycyny Doświadczalnej
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2002
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vol. 56
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issue 4
439-460
EN
The ABO blood group system was discovered one hundred years ago; the respective ABH antigens were found in the protein and lipid membrane components of various cells (most importantly, erythrocytes) and soluble blood group substances. The genetics of the ABO system was also elucidated. This old knowledge is still useful, bringing new applications. Although we do not really know the complete biological role of blood group systems, including that of the major ABO system, data, which is still being accumulated, ensure scientists and physicians of its imoprtance in research and the diagnosis and therapy of diseases. For example, susceptibility to some diseases has been shown to be connected with the blood group of the patient, or the phenotype changes of the ABH determinants may indicate the presence of defined form of cancer. The data presented in this article clearly suggest the validity of the ABO blood group system in medical and biological sciences.
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