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1

Statistical methods for microarray assays

100%
Krajewski P., Bocianowski J.
Journal of Applied Genetics
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2002
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vol. 43
|
issue 3
269-278
EN
The paper shortly reviews statistical methods used in the area of DNA microarray studies. All stages of the experiment are taken into account: planning, data collection, data preprocessing, analysis and validation. Among the methods of data analysis, the algorithms for estimating differential expression, multivariate approaches, clustering methods, as well as classification and discrimination are reviewed. The need is stressed for routine statistical data processing protocols and for the search of links of microarray data analysis with quantitative genetic models
2

Trypsin inhibitor electrophoretic patterns in Vicia faba L. and related species

81%
Zimniak-Przybylska Z., Przybylska J., Krajewski P.
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1999
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vol. 40
|
issue 4
283-292
EN
The studied material covered 58 accessions representing Vicia faba of section Faba, V. bithynica of section Bithynicae and seven species of section Narbonensis. Proteins of individual seeds were separated by polyacrylamide gel electrophoresis and the gels were stained for inhibitory activity against trypsin. The number of trypsin inhibitor (TI) bands recorded in particular species varied from three in V. eristalioides and V. galilaea to 15 in V. narbonensis; in total, 30 bands were distinguished in the examined material. Except for V. eristalioides, the studied species showed intraspecific variation with respect to electrophoretic TI patterns. A statistical analysis of the data, using hierarchical UPGMA grouping, resolved the studied taxa into three main clusters. V. faba subspecies/varieties formed one cluster. The second cluster consisted of V. narbonensis varieties and the rather distantly related V. eristalioides. The third cluster comprised V. bithynica as a peripheral species and the more closely associated V. galilaea, V. hyaeniscyamus, V. johannis, V. kalakhensis and V. serratifolia. The obtained results are discussed with reference to taxonomic relationships of the species under study.
3

Electrophoretic seed albumin patterns in the Vicia sativa L. aggregate

81%
Przybylska J., Zimniak-Przybylska Z., Krajewski P.
Journal of Applied Genetics
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2000
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vol. 41
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issue 3
139-149
EN
Electrophoretic analysis of seed albumins covered 201 accessions representing six subspecies of Vicia sativa L.: nigra, amphicarpa, incisa, sativa, macrocarpa and cordata. At least five individuals of each accession were examined separately. All the taxa under study showed variation with respect to albumin banding patterns, which was due to differences between accessions and individual variation within accessions. The number of albumin bands distinguished in particular taxa varied from 13 to 19. The statistical analysis of the electrophoretic data consisted in hierarchical grouping by the UPGMA method based on EUCLIDEAN distances. In the case of subsp. nigra and subsp. sativa, accessions originating from North Africa tended to form a group showing some distinctness from the remaining accessions. As to relationships among the studied taxa, subsp. nigra and subsp. cordata were shown to be the most closely related and their affinities to other members of the V. sativa aggregate were decreasing in the following order: subsp. macrocarpa, subsp. sativa, subsp. amphicarpa, subsp. incisa. The obtained results are discussed with reference to taxonomic relationships among the members of the V. sativa aggregate.
4
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Structural Stability of the High-Aluminium Zinc Alloys Modified with Ti Addition

71%
Krajewski W., Zak P., Orava J., Greer A., Krajewski P.
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issue 1
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