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EN
Ore flotation residue has been tested for the recovery of titanium in two different systems (agitated vessel and percolated fixed-bed column). For each of these systems, titanium extraction did not exceed 1 % of the metal available. Biomass removed from ore residue adsorbed a small part of the titanium with sorption capacities below 20-30 mg g-1, but the best part of this biomass was sequestered on the ore residue. Oxygen and carbon dioxide concentrations were monitored, and changes in concentration correlated with bacteria and fungal development.
EN
Absidia orchidis can be used as a source of several enzymes, amongst which chitosanase is one of the most interesting. Chitosanase hydrolyses the links between the mers of glucosamine or between mers of glucosamine and N-acetylglucosamine in the chains of chitosan and chitin. The aim of the presented work was the preliminary investigations of the chitosanase from the fungus Absidia orchidis. This chitosanase is an intracellular enzyme with molecular weight approx. 36 000 Da. The optimal conditions for a hydrolysis of chitosan were pH 4.5 and temperature 25C. This enzyme is stable at the optimal temperature for 24-48 hours, but after 7 days it was inactivated.
EN
The method of chitosan separation from fungus cell walls was presented by White, Farina, Fulton in 1979. Since that time many other investigators worked on that subject. None of them succeeded in developing a technology that would be worth-while as faras the economical aspect of the matter is concerned. The yield of chitosan from fungal mass or from cultivation medium depends on several factors like: strain of fungi used, cultivation method (submerged cultures or solid-state cultures), cultivation parameters (pH, temperature, time of cultivation). The aim of this paper was to compare the literature and own data presenting the influence of culture conditions on chitosan yields.
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