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Genetica Polonica
|
1994
|
vol. 35
|
issue 4
255-269
EN
Festuca pratensis Huds, occuring in nature as a diploid (2n=14) has been an object of many artificial polyplodization attemps.In present study an attempt has been made to select spontanous polyploids of F.pratensis from twin embryos of diploid meadow fescue varieties and to compare them with the colchitetraploid cv.Westa (2n=28).The studied material was found to have a low per cent (0.07) of seeds with twin embryos.It was found that 96.00% of twin plants were diploids.The most frequent among spontanous polyploids were triploids (3.46%), less frequent - tetraploids (0.47%) and pentaploids (0.08%).No haploids were found.The obtained spontaneous triploids and tetraploids, as compared to the colchitetraploid cv.Westa, were charcterized by a significant variation of their morphological traits.It was found that in natural tetraploids from twin-embryonic seeds of F.pratensis, polyploidization has no influence on the tillering ability decrease and plant organ enlargement observed in the colchitetraploid cv.Westa.
EN
Twelve Polish spring wheat cultivars and 18 spring wheat accessions from CIMMYT, Mexico, were examined for resistance to a highly pathogenic Fusarium culmorum strain KF846 and powdery mildew in 5-year field experiments. Resistant wheat cultivars (Sumai 3 and Frontana) served as controls. The mean percentage of Fusarium-damaged kernels (% FDK) for 5 years was lower in CIMMYT accessions (16.7%) than in Polish spring cultivars (28.3%). In all Polish spring cultivars, % FDK was higher than in the control cultivars Sumai 3 and Frontana (12?20%). The mean disease score (on a scale of 1?9) for powdery mildew (natural infection) for all examined cultivars and lines ranged from 0 to 7 and in the Polish spring cultivars was significantly lower (0?5). Cultivars Eta, Henika, Ismena, Jasna and Olimpia were found to be the least susceptible to powdery mildew in field experiments. The laboratory host-pathogen tests with Blumeria graminis f. sp. tritici isolates showed that only two cultivars were characterized by identical resistance patterns as the standard differential lines with documented resistance genes. Cultivar Alkora had the gene Pm3d, and Henika had Pm5. The gene Pm3d was identified in cultivars Jasna and Eta in combination with another unknown gene/genes. Cultivars Santa and Torka had the gene Pm5 in combination with another unknown gene/genes. Four cultivars: Banti, Ismena, Olimpia and Sigma, showed resistance to all mildew isolates employed in a laboratory test. The accession IPG-SW-14 was the least susceptible to both pathogens (F. culmorum and powdery mildew) in all 5 years of experiments. This line is the best candidate for deriving new cultivars with improved resistance to fungal diseases.
EN
The valuable genes of Aegilops biuncialis, Ae. ovata, Ae. kotschyi, and Ae. variabilis were transferred to rye, by crossing Aegilops-rye amphiploids with tetraploid and diploid substitution rye. The C-banded karyotype of the BC1 and BC2 generations of amphiploids with 4x substitution rye and BC1 with 2x substitution rye showed great variation in chromosome number and composition. In the BC1 generation of amphiploids with 4x and 2x substitution rye, seed set success rate and germination rate varied depending on origin. However, plant sterility in all cross combinations of amphiploids with 4x and 2x substitution rye resulted in their elimination from further experiments in the BC3 and BC2 generations, respectively. In backcrosses of 4x substitution rye with amphiploids Ae. variabilis x rye 4x, fertile 4x rye plants containing Aegilops chromatin were produced in the BC2 generation.
EN
Spring wheat nursery accessions, including 18 spring wheat lines derived in CIMMYT, Mexico, and 12 spring wheat cultivars bred in Poland, along with cultivars Frontana and Sumai 3 as resistant controls, were examined for resistance to leaf rust under field conditions. Multipathotype tests with 16 different pathogen isolates were performed for postulation of Lr genes in Polish cultivars. Besides, STS markers for resistance genes Lr1, Lr9, Lr10, Lr24, Lr28, Lr37 were analysed in the studied cultivars and lines with Thatcher near-isogenic lines as positive controls. All Polish cultivars appeared to be susceptible to leaf rust. Ten of the CIMMYT nursery lines (IPG-SW: #7, 11, 14, 21, 22, 23, 27, 29, 30, 32) and cv. Frontana were resistant in the same environment and can be sources of resistance genes. Marker for the Lr10 gene was identified in 6 accessions (IPG-SW #14, 22, 23, 29, 30, 32) exhibiting resistance to leaf rust, whereas markers for Lr1 and Lr28 genes were observed in all the examined accessions. STS markers for Lr9, Lr24 and Lr37 genes were not identified in the investigated accessions.
EN
Thirty doubled haploid (DH) lines of barley derived from F1 of a cross between the six-rowed cultivar Pomo and two-rowed cultivar Maresi were examined for susceptibility to Fusarium seedling blight (SB) and head blight (FHB), measured by mycotoxin (nivalenol) content of kernels. RAPD (random amplified polymorphic DNA) polymorphism was analysed by using 53 decamer primers. Amplification products (APs) were 200 bp up to 2000 bp in size on average 5.7 per primer and the total number of APs was 284, 51.06% of which were polymorphic. Only 32 APs differentiated the examined DH lines ? 19 APs for nivalenol content of kernels and 13 for seedling resistance. DH lines segregated with continuous distribution of resistance to FHB and SB. At the seedling stage all DH lines exhibited lower susceptibility than parental cultivars, but in the adult stage only two lines (MP 2 and MP 7) appeared to be more resistant to FHB, i.e. accumulated in kernels a lower amount of mycotoxin than cultivars Maresi and Pomo.
EN
Barley doubled haploids (DH) were examined for their susceptibility to Fusarium head blight caused by Fusarium culmorum. DH lines were derived from F1 Maresi (two-rowed) ? Pomo (six-rowed) hybrids by the 'H. bulbosum' method. Doubled haploids, parental cultivars and F1 and F2 hybrids were inoculated with Fusarium culmorum (W.G.Sm.) Sacc., isolate KF350 under field conditions. The kernel infection score, number of kernels per ear, kernel weight per ear, 1000-kernel weight, and kernel fractions were recorded in inoculated and control plants. Samples of kernels were analysed for presence of nivalenol and deoxynivalenol. In the inoculated plants a reduction of kernel number, kernel weight per ear, 1000-kernel weight and percentage of plump kernels was observed. Generally, inoculation caused a significant decrease in the kernel fraction > 2.5 mm, and increase in the fractions 2.5-2.2 and < 2.2 mm. This tendency was more visible in 2-rowed than in 6-rowed lines. The nivalenol content of inoculated doubled haploids ranged from 0.16 to 7.61 mg/kg, whereas their deoxynivalenol content ranged from 0.000 to 0.253 mg/kg. Significant relationships between the kernel infection score and nivalenol content, kernel yield per ear, 1000-kernel weight and kernel fraction > 2.5 mm were observed. Transgression effects were noted in some DH lines, in which the reduction of kernel characters was lower than in parental cultivars. Doubled haploids with a positive and negative transgression for nivalenol and deoxynivalenol content were also recorded.
EN
The genetic determination of variability of barley doubled haploid (DH) lines in regard of their susceptibility to Fusarium head blight caused by Fusarium culmorum was studied. The susceptibility was evaluated in a 3-year field experiment on the basis of reduction in yield traits and mycotoxin accumulation in infected kernels. The following traits were analysed in inoculated and control plants: kernel number and weight per ear, 1000-kernel weight, percentage of plump kernels (>2.5 mm), deoxynivalenol (DON) content and nivalenol (NIV) content of kernels. On the basis of the obtained data, heritability coefficient (ratio of genotypic to phenotypic variance) was assesed, and genetic parameters as well as the number of effective factors were estimated. Heritability coefficients calculated from two-way analysis of variance, i.e. regarding the influence of years and year ? genotype interaction, appeared to be exceptionally low and ranged from 5.2% for the reduction in plump kernels to 38.2% for the reduction in 1000-kernel weight. In the case of mycotoxin accumulation about 60% of the observed variability in NIV concentration and 30% in DON concentration resulted from genetic differences among lines. Additive effects of genes were important for all the analysed traits. Significant effects of dominance and dominance ? dominance were observed for 1000-kernel weight and percentage of plump kernels. Moreover, it was found that the observed variability in yield trait reduction resulted from the segregation of 5-6 effective factors, DON content from 4 factors, while NIV content from 5 factors.
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