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ISOLATION AND CHARACTERIZATION OF CHITIN FROM SHELLS OF THE FRESHWATER CRAB POTAMON ALGERIENSE

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Fadlaoui S., El Asri O., Mohammed L., Sihame A., Omari A., Melhaoui M.

Progress on Chemistry and Application of Chitin and its Derivatives

2019

vol. 24

23 - 35

This work presents, for the first time, the extraction and characterization of chitin from the shell of the freshwater crab species Potamon algeriense with a standardized and revised chemical method. Chitin and chitosan were isolated following demineralization, deproteinization, decolouration (raw chitin), and deacetylation (chitosan). After boiling, drying, and grinding, 62.12% of the ground shell was obtained. A yield of 40.92% was obtained after demineralization of ground crab shell, while after the deproteinization process 8.74% was obtained. After decolourization, 8.27% of raw chitin was obtained, and the final amount of chitosan extracted from the crab shells was approximately 5.89%. We also characterized the isolated chitin by determining its physicochemical properties using X-ray powder diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA).

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1 Progress on Chemistry and Application of Chitin and its Derivatives

1 2019

1 El Asri O.

1 Fadlaoui S.

1 Melhaoui M.

1 Mohammed L.

1 Omari A.

1 Sihame A.

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ISOLATION AND CHARACTERIZATION OF CHITIN FROM SHELLS OF THE FRESHWATER CRAB POTAMON ALGERIENSE