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Presence of histamine and mast cells in chicken oviduct

100%

Hrabia A., Rzasa J., Paczoska-Eliasiewicz H., Slomczynska M.

Folia Biologica

2001

vol. 49

issue 3-4

265-271

The purpose of the present study was: (1) to demonstrate immunocytochemically the localization of histamine in the wall of four chicken oviductal parts, i. e. infundibulum, magnum, isthmus, and shell gland, (2) to identify the presence of mast cells in chicken oviduct, and (3) to determine histamine concentration in oviductal tissue by the spectrofluorometric method. Experiments were carried out on Isa Brown laying hens decapitated just after oviposition. The specific immuno-reactivity for histamine and the presence of mast cells were found in the wall of all the examined oviductal parts. The immuno-reactive histamine was localized in epithelium, tubular glands, connective tissue layer, circular and longitudinal muscles, and endothelium and muscles of blood vessels. The intensity of immuno-positive reaction was as follows: infundibulum > shell gland > magnum = isthmus and correlated with quantitatively determined histamine level and tissue density of mast cells. It is suggested that mast cells are the main source of histamine in the chicken oviduct.

Androgens and FSH affect androgen receptor and aromatase distribution in the porcine ovary

88%

Slomczynska M., Szoltys M., Duda M., Sikora K., Tabarowski Z.

Folia Biologica

2003

vol. 51

issue 1-2

63-68

In the present study the authors investigated whether androgens could interact with FSH to induce aromatase and androgen receptor expression in porcine granulosa cells. Dissected whole porcine follicles (small, medium, and large) were incubated for 8 hours in M199 medium supplemented with testosterone (10-7 M), FSH (100 ng/ml) or both those hormones. After incubation, the follicles were fixed and immunostained to visualise androgen receptor and aromatase. In cultures of granulosa cells isolated from small and large follicles, oestrogen secretion was measured by appropriate RIA. Incubation of follicles with testosterone and FSH increased aromatase immunoreactivity in preantral and early antral (i.e. small) follicles. The immunostaining for androgen receptor was slightly higher in medium follicles, while such hormonal stimulation had no effect on small and large follicles. Moreover, granulosa cells isolated from small follicles cultured with both testosterone and FSH produced more estradiol than control cultures (40 pg vs. 100 pg/105cells). The level was relatively close to that obtained in the culture of control granulosa cells isolated from large preovulatory follicles (105 pg/105 cells). These results indicate that testosterone acts synergistically with FSH to increase aromatase expression in the small porcine follicles.

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2 Folia Biologica

1 2003

1 2001

2 Slomczynska M.

1 Duda M.

1 Hrabia A.

1 Paczoska-Eliasiewicz H.

1 Rzasa J.

1 Sikora K.

1 Szoltys M.

1 Tabarowski Z.

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Presence of histamine and mast cells in chicken oviduct

Androgens and FSH affect androgen receptor and aromatase distribution in the porcine ovary