Search results

Sort By:

Limit search:

The effects of a 4-week vibrotherapy programme on the reduction of adipose tissue in young women with cellulite - a pilot study

100%

Pilch W., Nastałek M., Piotrowska A., Czerwińska-Ledwig O., Zuziak R., Maciorowska A., Golec J.

Medical Rehabilitation

2018

vol. 22(4)

18-24

Wprowadzenie: Lipodystrofia jest problemem o dużym nasileniu u współczesnych kobiet. Do tej pory nie opracowano w pełni skutecznych metod terapeutycznych całkowicie niwelujących tego typu zmiany. Mało poznaną i jak do tej pory nie badaną metodą w profilaktyce i niwelowaniu objawów lipodystrofii może być masaż wibracyjny. Cel pracy: Celem pracy była ocena skuteczności wibroterapii na zmiany lipodystroficzne. Materiał i metoda: W badaniach uczestniczyło 10 wolontariuszek ze zmianami cellulitowymi w wieku 21-23 lata o określonej aktywności fizycznej mierzonej międzynarodowym kwestionariuszem IPAQ. U wszystkich osób włączonych do badania dokonano oceny żywienia na podstawie 5-dniowej analizy żywienia, wykonano pomiar obwodów talii, bioder i ud, oszacowano skład ciała za pomocą bioimpedancji oraz wykonano zdjęcia z dermatoskopu. Zastosowano 4 tygodniową wibroterapię oscylacyjno-cykloidalną z zastosowaniem Rehabilitacyjnego Aparatu Masującego Vitberg+ (Nowy Sącz). Badania wykonano 2-krotnie, przed i po zastosowanej 4 tygodniowej wibroterapii. Wyniki: Po 4 tygodniowej serii zabiegów wibracyjnych zaobserwowano średnie zmniejszenie obwodów ud (Δ1,55 cm, p=0,002 dla uda prawego, Δ1,50 cm, p=0,000 dla lewego), bioder (Δ1,25 cm, p=0,006) oraz talii (Δ2,30cm, p=0,002). Zaproponowana seria zabiegowa wywołała redukcję masy tkanki tłuszczowej całego ciała (Δ0,42 kg, p=0,009) bez wpływu na zawartość wody w organizmie, a analiza wyników obrazowania dermatoskopowego ujawniła poprawę ukrwienia skóry. Wniosek: Wibroterapia oscylacyjno-cykloidalna może być skutecznym narzędziem niwelowania zmian lipodystroficznych.

Introduction: Lipodystrophy is a common problem for modern women. So far, no fully effective therapeutic methods have been developed to eliminate this type of change. Vibration massage is a method that has not yet been studied in the prevention and elimination of lipodystrophy symptoms. Aim of the study: The aim of this study was to evaluate the effectiveness of vibrotherapy on the reduction in lipodystrophic changes. Material and method: 10 volunteers with cellulite changes at the age of 21-23 with physical activity levels described by the international IPAQ questionnaire participated in the study. All women included in the study were evaluated on the basis of 5-day nutrition analysis. Before and after 4 weeks of vibrotherapy, waist, hip and thigh circumferences were measured, and body composition was assessed using bioimpedance and dermatoscope photos were taken. Oscillating cycloidal vibration was generated by the Vitberg+ Rehabilitation Device (Nowy Sącz). Results: After the 4-week series of vibrations, the following average reduction in thigh circumference was observed (Δ1.55 cm, p = 0.002 for right thigh, Δ1.50 cm, p = 0.000 for left), hips (Δ1.25 cm, p = 0.006) and waist (Δ2.30 cm, p = 0.002). The proposed treatment series caused a reduction in body fat mass (Δ0.42 kg, p = 0.009) without affecting the body’s water content, and the analysis of dermatoscopic imaging results revealed improvement in skin perfusion. Conclusion: Oscillating-cycloidal vibration therapy can be an effective tool to alleviate lipodystrophic changes

Optimization of Western blotting analysis for the isolation and detection of membrane xenobiotic transporter ABCG2

100%

Szczygieł M., Markiewicz M., Szafraniec M., Zuziak R., Urbańska K., Fiedor L.

Acta Biochimica Polonica

2017

vol. 64

issue 3

437-443

All organisms are exposed to numerous stress factors, which include harmful xenobiotics. The diversity of these compounds is enormous, thus in the course of evolution diverse biological defense mechanisms at various levels of organization have developed. One of them engages an evolutionarily conserved family of transporters from the ABC superfamily, found in most species - from bacteria to humans. An important example of such a transporter is the breast cancer resistance protein (BCRP/ABCG2), a typical integral membrane protein. It plays a key role in the absorption, distribution and elimination of a wide variety of xenobiotics, including drugs used in chemotherapy, and is involved in multidrug resistance. It also protects against phototoxic chlorophyll derivatives of dietary origin. BCRP is a hemitransporter which consists of one transmembrane domain, made of six alpha-helices forming a characteristic pore structure, and one ATP-binding domain, which provides the energy from ATP hydrolysis, required for active transport of the substrates. The isolation of BCRP is still not an easy task, because its insolubility in water and the presence of membrane rafts pose serious methodological and technical challenges during the purification. The aim of this study was to optimize the methods for detection and isolation of BCRP-enriched fractions obtained from animal tissue samples. In this report we describe an optimization of isolation of a BCRP-enriched membrane fraction, which is suitable for further protein quantitative and qualitative analysis using the molecular biology tools.

Refine search results

1 Acta Biochimica Polonica

1 Medical Rehabilitation

1 2019

1 2017

2 Zuziak R.

1 Czerwińska-Ledwig O.

1 Fiedor L.

1 Golec J.

1 Maciorowska A.

1 Markiewicz M.

1 Nastałek M.

1 Pilch W.

1 Piotrowska A.

1 Szafraniec M.

1 Szczygieł M.

1 Urbańska K.

Search results

The effects of a 4-week vibrotherapy programme on the reduction of adipose tissue in young women with cellulite - a pilot study

Optimization of Western blotting analysis for the isolation and detection of membrane xenobiotic transporter ABCG2