Search results

Sort By:

Limit search:

A novel laccase from fresh fruiting bodies of the wild medicinal mushroom Tricholoma matsutake

100%

Xu L., Zhu M., Chen X., Wang H., Zhang G.

Acta Biochimica Polonica

2015

vol. 62

issue 1

35-40

The knowledge about biological activities of constituents from medicinal mushrooms belonging to the genus Tricholoma is limited. A 59-kDa laccase has now been purified from fresh fruiting bodies of the mushroom Tricholoma matsutake. The purification protocol entailed ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on CM-cellulose, affinity chromatography on ConA-Sepharose, and gel filtration by fast protein liquid chromatography on Superdex 75. Of the various affinity and ion exchange chromatographic media employed, the laccase bound only on Con A-Sepharose. The activity of the laccase did not undergo major changes over the temperature range 20-80°C. However, all activity vanished following exposure to 100°C for 10 minutes. The enzyme activity varied only slightly over the pH range 3-5, with the optimal pH of 5, but exhibited a precipitous decline when the pH was increased to 6, and was undetectable at pH 8 and 9. The laccase showed activity in the decolorization of azo dyes without a mediator. Its N-terminal sequence demonstrated only slight resemblance to those of other mushroom laccases. The newly described laccase is distinctive from the previously isolated Tricholoma mushroom laccases in a number of aspects.

Cadmium exposure induced itai-itai-like syndrome in male rats

86%

Chen X., Zhu G., Jin T., Gu S., Tan M., Xiao H., Qiu J.

Open Medicine

2011

vol. 6

issue 4

425-434

Fourteen Sprague-Dawley male rats were randomly divided into 2 groups which were given CdCl2 at the doses of 0 and 1.5 mg /kg for 12 weeks. Before sacrifice, microCT scanning were performed on the proximal tibia and urine were collected for cadmium and N-acetyl-beta-D-glucosaminidase assay, then all of rats were sacrificed and blood was collected for biomarkers measurement; bone tissues were collected for bone mass, histology and biomechanical analysis. The cadmium in blood, urine, bone and kidney of rats treated with cadmium was significantly higher than those in the control group. The bone mineral density, and bone mineral ability of rats treated with cadmium were obviously decreased by 20%–50% compared to controls. Bone microstructure index and trabecular separation of rats treated with cadmium were obviously lower (−50%) and significantly higher (+150%) than that in the control group. Bone biomechanical property decreased by 30%–60% in cadmium treated rats compared to control. Tartrate-resistant acid phosphatase 5b and alkaline phosphatase levels of rats treated with cadmium were significantly higher than those in control, but serum osteocalcin level decreased greatly by cadmium. Obvious proximal tubule damage occurred after cadmium exposure. These observations gave clear and comprehensive evidence that cadmium exposure could induce itai-itai-like syndrome in male rats.

Refine search results

1 Acta Biochimica Polonica

1 Open Medicine

1 2015

1 2011

2 Chen X.

1 Gu S.

1 Jin T.

1 Qiu J.

1 Tan M.

1 Wang H.

1 Xiao H.

1 Xu L.

1 Zhang G.

1 Zhu G.

1 Zhu M.

Search results

A novel laccase from fresh fruiting bodies of the wild medicinal mushroom Tricholoma matsutake

Cadmium exposure induced itai-itai-like syndrome in male rats