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Monocytes in children with leukemias and lymphomas - down-regulation of HLA and costimulatory molecules.

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Łuczyński W., Stasiak-Barmuta A., Krawczuk-Rybak M., Szymański M., Malinowska I., Mitura-Lesiuk M.

Acta Biochimica Polonica

2004

vol. 51

issue 4

1067-1073

The aim of the study was to evaluate the function of monocytes in children with leukemias and lymphomas based on the expression of critical costimulatory, activatory and adhesion molecules (CD80, CD86, HLA-DR and CD54 = ICAM-1), estimated with tricolor flow cytometry. In comparison to the control group we found a lower percentage of monocytes with costimulatory molecules (CD80 before and CD86 after lipopolysaccharide stimulation) at the time of diagnosis and of monocytes with HLA-DR molecules after remission induction. We also noted a lower percentage of monocytes with HLA-DR expression in the group with severe or therapy resistant infections. The results of our investigation suggest some defect in costimulation and antigen presentation in lymphoproliferative diseases in children.

CD40 stimulation induces differentiation of acute lymphoblastic leukemia cells into dendritic cells

84%

Łuczyński W., Stasiak-Barmuta A., Iłendo E., Krawczuk-Rybak M., Malinowska I., Mitura-Lesiuk M., Parfieńczyk A., Szymański M.

Acta Biochimica Polonica

2006

vol. 53

issue 2

377-382

Despite the very high percentage of long-term remissions in acute lymphoblastic leukemia (ALL) in children, some of them suffer from recurrence of the disease. New treatment modalities, e.g. effective geno- and immunotherapy are needed. The use of neoplasmatic cells to present tumor antigens is one of the approaches in cancer vaccines. ALL cells lack the expression of costimulatory molecules and are poor antigen presenting cells (APCs) for T-cell activation. CD40/40L interaction stimulates B-cells to proliferate, differentiate, upregulate costimulatory molecules and increase antigen presentation. The aim of the study was to test the hypothesis that ALL cells can be turned into professional APCs by CD40L activation. Children with B-cell precursor ALL were enrolled into the study. Mononuclear cells from bone marrow or peripheral blood were stimulated with CD40L and interleukin 4. Results: 1) after culture we noted upregulation of all assessed costimulatory, adhesion and activatory molecules i.e. CD1a, CD11c, CD40, CD54, CD80, CD83, CD86, CD123, HLA class I and II; 2) CD40L activated ALL cells induced proliferation of allogeneic T-cells (measured by [3H]thymidine incorporation). These results confirm the possibility of enhancing the immunogenicity of ALL cells with the CD40L system and indicate that this approach can be used in immunotherapeutic trials.

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2 Acta Biochimica Polonica

1 2006

1 2004

2 Krawczuk-Rybak M.

2 Malinowska I.

2 Mitura-Lesiuk M.

2 Stasiak-Barmuta A.

2 Szymański M.

2 Łuczyński W.

1 Iłendo E.

1 Parfieńczyk A.

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Monocytes in children with leukemias and lymphomas - down-regulation of HLA and costimulatory molecules.

CD40 stimulation induces differentiation of acute lymphoblastic leukemia cells into dendritic cells