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1

Reversible inhibition of movement in the amoebae Dictyostelium discoideum and its effect on chemoattractant recognition

100%
Waligorska A., Wianecka-Skocze? M., Korohoda W.
Folia Biologica
|
2008
|
vol. 56
|
issue 3-4
123-133
EN
The cell fixatives formaldehyde and KMnO4 at low concentrations reversibly inhibit the movement of D. discoideum amoebae without directly interfering with cell viability. This inhibition of cell movement is accompanied by the decreased attachment of cells to substratum.When the tenacity and attachment of immobilized cells are artificially increased by compressing cells between two glass surfaces, the amoebae begin to move even in the presence of the fixatives. Amoebae starved for 24 hours, subjected to fixatives and a mineral salt solution in which they remained motionless, maintained chemotactic res acid and only after a few hours of active locomotion became reactive to cAMP.
2

Some difficulties in research into cell motile activity under isotropic conditions

100%
Waligorska A., Wianecka-Skoczen M., Nowak P., Korohoda W.
Folia Biologica
|
2007
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vol. 55
|
issue 1-2
9-16
EN
Movement of Dictyostelium discoideum amoebae under isotropic and anisotropic conditions was recorded and analysed with computer-aided methods and the results are presented in various manners as described in the subject literature. Cell movement under isotropic conditions showed great diversity. Some cells moved almost in a straight path whereas others in close proximity turned around with little net translocation. When cell movement under isotropic conditions was observed, no direct correlation was found between the total length of cell trajectories and the length of final displacements of the cells. It was necessary to present the results in the form of histograms, circular diagrams of cell trajectories or in scatter correlation diagrams showing the motile behaviour of many individual cells. These methods of presentation are more informative than methods which present only average values, the 'representative' behaviour of single cells, or start and end points of cell tracks. The latter methods can only illustrate but do not document the results of experiments. The use of statistical methods appears necessary in cases when it is difficult to monitor the same cells before and during experimental treatment. However, when cell movement under anisotropic conditions becomes oriented and ordered as during tactic cell movements, then the diversity in cell behaviour decreases and methods based on estimation of starting and end points of cell positions appear more credible.
3

Motile activities of Dictyostelium discoideum differ from those in Protista or vertebrate animal cells

100%
Waligorska A., Wianecka-Skoczen M., Korohoda W.
Folia Biologica
|
2007
|
vol. 55
|
issue 3-4
87-93
EN
Cell movement in the amoebae Dictyostelium discoideum has been examined in media differing in monovalent cation concentration (i.e. Na+ and K+). Under isotonic or even slightly hypertonic conditions, the cells move equally well in solutions in which either potassium or sodium ions dominate. However, in strongly hypertonic solutions the amoebae showed motility in a 2% potassium chloride solution, but remained motionless in a hypertonic 2% sodium chloride solution. This inhibition of D. discoideum amoebae movement in a hypertonic sodium chloride solution was fully reversible. Such behaviour corresponds to that of plant, fungi, and some invertebrate animal cells rather than protozoan or vertebrate cells. These observations suggest that studies using D. discoideum as a model for cell motility in vertebrate animal tissue cells should be considered with caution, and would seem to confirm the classification of cellular slime moulds as related rather to Fungi than to Protista. This also shows that the cell membrane models should consider the asymmetry in sodium /potassium ion concentrations found in vertebrate animal cells as one of various possibilities.
4

Comparison of proliferation and motile activity between human keratinocytes isolated from skin and oral muco

76%
Drukala J., Zarzecka J., Gojniczek K., Waligorska A., Zapala J., Korohoda W.
Folia Biologica
|
2005
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vol. 53
|
issue 1-2
21-28
EN
Objective: Epithelial wound repair assures the recovery of the epithelial barrier after wounding. During wound healing epithelial cells migrate to cover the wound surface. For healing of skin wounds the skin keratinocytes can be replaced by oral mucosa epithelial cells grown in vitro. The presented experiments were carried out in order to compare the proliferation, morphology, and migration between human keratinocytes isolated from human skin and oral mucosa. Materials and methods: Human epidermal and oral mucosa keratinocytes from primary culture were used in all experiments. Cell motility and shape were determined using computer-aided methods. Results and conclusions: It was demonstrated that although both cell types exhibit the same typical epithelial morphology, oral mucosa keratinocytes locomote significantly faster than skin keratinocytes. They also differ in proliferation activity. Oral mucosa keratinocytes exhibited faster growth and different actin cytoskeleton organisation than skin keratinocytes under in vitro conditions. Autologous oral mucosa keratinocytes may be expanded in vitro and used for skin wound healing in vivo.
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