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Bioassays were made in the laboratory with fry of freshwater teleosts Cyprinus carpio (common carp) and Hypophthalmicthys molitrix (silver carp) and the oligochaete worm Branchiura sowerbyi. Acclimation of these organisms by 100-150 ppm lime for 96 h could significantly reduce their susceptibility to cadmium (Cd). An increase in the dose of lime or period of acclimation could further reduce the susceptibility of these organisms, but the upper limit of the dose and period of acclimation varied with the species of organisms. Maximum doses of lime that could be used for acclimation of common carp fry, silver carp fry, and worms were 450 mg/l for 96 h and 100 mg/l for 12 days, 400 mg/l for 96 h and 100 mg/l for 8 days, and 200 mg/l for 96 h and 75 mg/l for 8 days respectively. The minimum effective doses of lime for acclimation of these organisms were respectively 150, 100, and 75 mg/l for 96 h. For further reduction of susceptibility an increase of at least 100 mg/l of lime was necessary for fish and 50 mg/l was necessary for worms.
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