Full-text resources of PSJD and other databases are now available in the new Library of Science.
Visit https://bibliotekanauki.pl
Preferences help
Polish version English version Language
enabled [disable] Abstract
Number of results

Results found: 2

Number of results on page
first rewind previous Page / 1 next fast forward last

Search results

help Sort By:

help Limit search:
first rewind previous Page / 1 next fast forward last
1

Rooting in vitro and acclimatization in the greenhouse of herbaceous peony plantlets

100%
Gabryszewska E., Kawa-Miszczak L.
Biotechnologia
|
2010
|
issue 2
172-179
EN
The influence of sucrose concentration (10 and 30 g l-1), low level of nitrogen salts (1/8 KNO3, 1/8 NH3NO4), auxins (IBA 1 mg l-1 + IAA 1 mg l-1 + NAA 0,01 mg l-1), and temperature (15?C, 20?C) on the rooting in vitro and acclimatization in the greenhouse of two cultivars ('Jadwiga', 'Professor Wojcicki') of Paeonia lactiflora were investigated. The level of endogenous carbohydrates in the peony shoots and roots during the rooting and acclimatization phases were analysed. There were a higher rooting and acclimatization percentage of 'Professor Wojcicki' cultivar than 'Jadwiga' cultivar . Also, more roots were produced by shoots of 'Professor Wojcicki' cultivar . The presence of auxins in the medium and the higher concentration of sucrose (30 g l-1) stimulated number of roots/shoot. On the other hand, the higher rooting percentage was found on the auxin?free medium in the presence of high level of sucrose. The shoots of 'Professor Wojcicki' rooted best when cultured at 20?C, but the shoots of 'Jadwiga' at lower temperature - 15?C. During the rooting stage, the major sugars detected in the peony microplants were sucrose, glucose and fructose. After four weeks of acclimatization, the plantlets accumulated starch and showed strong inhibition of shoot growth, and dormant buds were developed.
2

Effect of meta-topolin on the growth and development of Pelargonium ? hortorum shoots in vitro

100%
Wojtania A., Gabryszewska E.
Biotechnologia
|
2004
|
issue 2
162-167
EN
The influence of BAP (0.1; 0.5 mg l-1) and meta-topolin (mT) (0.2; 1.0 mg l-1) added alone or together with IBA (0.02 mg l-1), on the growth and development of shoot tips and axillary buds of P. ? hortorum 'Bargpalais' isolated in April and July was compared. The explants taken in April had higher regeneration ability. Both cytokinin stimulated growth of buds, but shoots of good quality (without deformation and vitrification symptoms) were noted on the medium supplemented with mT (0.4 mg l-1). Application of mT (1.0 mg l-1) in the multiplication stage resulted in better shoots quality as well as more effective shoots formation (4.8-7.1, depending on subculture) compared to BAP. The observation of 4 subsequent subcultures after initiation of cultures showed that the quality of shoots P. ? hortorum grown in the presence of BAP decreased with time.
first rewind previous Page / 1 next fast forward last
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.