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Odkrycie struktury komórkowej tkanki nerwowej

100%
Ślósarek G.
Kosmos
|
2008
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vol. 57
|
issue 1-2
103-107
EN
A great breakthrough in development of biological sciences was the cell theory formulated in the first half of the 19th century. It permitted a reduction of the studies of a huge diversity of living organisms to analysis of a single animal or plant cell. Till the end of the 19th century the nervous tissue was not described in terms of this theory, because a simple optical microscope was an insufficient instrument when applied in the studies of such complex but fascinating structure. The implementation of the new method of staining proposed by Golgi - reazione nera, opened the possibility of structural investigation of the nervous tissue. Camillo Golgi and Santiago Ramon y Cajal were the first to apply this method in cytological studies. They worked separately, on the same set of data, but the interpretations presented by each of them led to substantially different conclusions. The work of Cajal has become fundamental for development of the neuron theory, which - together with the postulate of the functional localization - is basic for the current knowledge of the structure and function of the nervous system. Therefore, Santiago Ramon y Cajal has been recognized as the precursor of the research discipline known as neuroscience.
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Denaturation and aggregation of lysozyme in water-ethanol solution

51%
Szymańska A., Hornowski T., Ślósarek G.
Acta Biochimica Polonica
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2012
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vol. 59
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issue 2
317-322
EN
We have applied rheological methods for the analysis of ethanol-lysozyme interaction during the process of denaturation and aggregation of the protein. At low concentration of ethanol a destruction of the hydration shell of lysozyme is observed. With the increase in the ethanol concentration a structural transformation takes place. It leads to the formation of a protein aggregate with an elongated structure. The rheological characteristics of lysozyme-water-ethanol solution changes from Newtonian to pseudoplastic.
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