At present, the genus Trichinella comprises eleven species of five encapsulated muscle larvae (T. spiralis, T. nativa, T. britovi, T. nelsoni and T. murrelli), three not encapsulated (T. pseudospiralis, T. papuae and T. zimbabwensis) and three genotypes of nonspecific status (T6, T8, T9). The diagnostic characteristics of these species are based on biological, biochemical and genetic criteria. In Poland two of these genotypes T. spiralis and T. britovi are present. The distribution in Poland of Trichinella species in wild animals, red foxes, wild boars, and domestic pigs is presented. Muscles, mainly diaphragm and forelegs, were collected from animals killed by hunters in different regions of Poland between 1996-2004, and Trichinella were collected after artificial digestion of muscles. and preserved in absolute alcohol until molecular identification. Of the 21 Trichinella isolates from domestic pigs 1 was obtained from T. britovi, 20 from T. spiralis. Of the 53 Trichinella isolates from red foxes 40 were obtained from T. britovi, 6 from T. spiralis, 4 were after mixed infection with two species and 3 from not identified ones. Of the 92 Trichinella isolates from wild boars 65 were obtained from T. spiralis, 18 from T. britovi, 2 after mixed infection and 6 were not identified. Additionally, 3 T. britovi isolates were from wolves from Bieszczady, 3 T. spiralis isolates were obtained from raccoon dogs and 1 T. spiralis isolate was from marten. Molecular techniques (DNA analysis) confirmed that two Trichinella species: T. spiralis and T. britovi are involved in the epidemiology of trichinellosis among humans in Poland.
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