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1

Genetically engineered potato resistant to potato leafroll virus infection

100%
Palucha A., Chrzanowska M., Zagorski W., Hulanicka D.
Biotechnologia
|
1997
|
issue 4
38-47
EN
Binary plasmids carrying different fragments of the coat protein gene of potato leafroll luteovirus were constructed for transformation of commercial potato cultivar Bzura. Several transgenic plant lines were obtained and characterised. The resistance of transgenic plants to the virus was tested by inoculation with aphids or grafting. Selected transformants expressing viral RNA were resistant to virus challenge by viruliferous aphids. Expression of the antisense RNA prevented virus infection even after grafting with scions from infected plants.
2

Endonuclease restriction of SCAR amplicons SC811 is required to identify Ns-false-positive markers in PVS-susceptible potato cultivars

88%
Szajko K., Chrzanowska M., Strzelczyk-Zyta D., Zagorska H., Marczewski W.
Journal of Applied Genetics
|
2008
|
vol. 49
|
issue 1
45-47
EN
The Ns gene confers resistance of potato to Potato virus S (PVS). Sixteen German and Dutch potato cultivars, all registered in Poland, were found to be susceptible to PVS infection. However, scoring of the cultivars for the presence of the Ns-linked SCAR marker SC811454 revealed additional amplicons with a similar electrophoretic migration rate as that of SC811454, which resulted in ambiguous determination of the genotype at the Ns locus. MboI or FokI treatment of the PCR products allowed to detect their Ns-unspecificity in PVS-susceptible potato cultivars.
3

Potato and tobacco cultivars transformation towards potato virus Y resistance

76%
Chachulska A. M., Chrzanowska M., Flis B., Krzymowska M., Lipska-Dwuznik A., Robaglia C., Zagorski W.
Biotechnologia
|
1997
|
issue 4
48-54
EN
In recent years a new subgroup of necrotic potato virus Y (PVY) isolates has spread in the Polish fields. To counter this infection, the PVY resistance was introduced into selected potato and tobacco cultivars. Plants were transformed by agroinfection with pROK2 derived binary plasmids, carrying an appropriate fragment of the PVY genome (patent applied for). Kanamycin resistant plants were screened by PCR for the presence of PVY cDNA inserts, positive transformants were tested for virus resistance by inoculation with sap from infected plants, followed by observations of disease symptoms and virus accumulation analysis. Several resistant potato and tobacco clones were identified and their phenotypes were preliminarily characterised.
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