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  1. 1 World News of Natural Sciences

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  1. 1 2025

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  1. 1 Khusro A.

  2. 1 Prabu R. V.

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Characterization and sequence analyses of hyper-cellulase producing Bacillus paramycoides

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Khusro A., Prabu R. V.
World News of Natural Sciences
|
2025
|
vol. 61
|
issue 2
65-74
EN
The present study was investigated to isolate and identify a hyper-cellulase producing bacterium from soil. Initially, a total of 11 cellulase producing bacteria were isolated from the collected soil sample using serial dilution technique, followed by cellulose hydrolysis plate assay method. The hyper-cellulase producing bacterium was identified as Bacillus paramycoides strain KASHA using different biochemical and molecular characterization tools. Furthermore, 16S rRNA sequences of strain KASHA were used to determine the secondary structure of RNA using RNAfold web server. As per the results obtained, the minimum free energy value for strain KASHA was calculated as -199.69 kcal/mol. Mountain plot and entropy depicted the hierarchical representation of RNA secondary structure. The GC content of sequence for strain KASHA was calculated as 53%. The evolutionary relationship of the strain was determined using Neighbor Joining algorithm which showed close resemblance with other bacilli strains.
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