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The efficiency of immunoenzyme assay in the diagnosis of lambliosis

100%
Miladinovic Tasic N., Tasic S., Tasic I.
Open Medicine
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2010
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vol. 5
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issue 4
464-469
EN
The aim of this study is to determine the difference in efficiency of direct immunoenzyme-linked assay (EIA) and conventional microscopy (CM) plus conventional concentration technique (CCT) using comparative analysis in the diagnosis of symptomatic and asymptomatic lambliosis when only one stool sample is to be tested. The study enrolled 577 examinees: 208 patients and 369 asymptomatic examinees. Lambliosis was diagnosed using CM plus CCT (three stool samples) and direct EIA (the first sample). All statistical parameters of the EIA method were 100% in the patients with symptoms of infection. In addition to that, in the group of asymptomatic carriers of Giardia lamblia (G. lamblia) some very high values of these parameters were recorded too, with sensitivity and negative predictive value being both at 100%. In contrast to the EIA method, CM plus CCT of the first stool sample demonstrated significantly lower sensitivity (66.67%) compared to the reference standard. The study did not demonstrate any statistically significant differences in diagnostic efficiancy between the EIA testing of one stool sample and CM plus CCT (p<0.05). However, the observed difference in diagnostic efficiency between the methods was very close to the cut-off value for statistical significance (p=0.06).
2
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Asymptomatic giardiasis-more prevalent in refugees than in native inhabitants of the city of Nis, Serbia

76%
Miladinovic-Tasic N., Tasic S., Kranjcic-Zec I., Tasic G., Tasic A., Tasic I.
Open Medicine
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2008
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vol. 3
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issue 2
203-206
EN
Giardiasis is a parasitic infection of the digestive tract, most commonly occurring in closed communities such as schools, kindergartens, prisons, and campuses. The civil war in the former Yugoslav republics and in Kosovo caused a large number of refugees to take shelter in the territory of Serbia. Such large numbers of refugees could be accommodated only in the collective centers. Our aim was to examine the differences in the prevalence of asymptomatic giardiasis among 122 refugees from the former Yugoslav republics who lived in the collective centers in Nis, Serbia, and 241 native Nis inhabitants. Conventional microscopic examination (CME) of three stool samples with or without concentration technique and the enzyme immunoassay (EIA) methods were used. The CME method of three stool samples is considered the gold standard in our statistical survey. Asymptomatic giardiasis is found in 7 refugees (5.7%) using the EIA method, while using the CME (3 samples) Giardia duodenalis (G. duodenalis) was detected in 6 persons (4.9%). Using the EIA method and the CME (3 samples) G. duodenalis was detected in only 1 person in the population group of native inhabitants (0.4%). Asymptomatic giardiasis was more prevalent in the population group of refugees accommodated in collective centers than in native inhabitants in the Nis municipality, Serbia.
3
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Impact of depression on sexual dysfunction and HRQoL in CAD patients

64%
Tasic I., Lazarevic G., Kostic S., Djordjevic D., Simonovic D., Rihter M., Vulic D., Stefanovic V.
Open Medicine
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2014
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vol. 9
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issue 1
54-63
4
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Relation between bone density and certain parameters of lipid status in postmenopausal women

64%
Dimic A., Popovic M., Tasic I., Djordjevic D., Stojanovic S., Stamenkovic B., Popovic D., Milenkovic S., Dimic M., Nedovic J.
Open Medicine
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2012
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vol. 7
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issue 5
642-649
EN
The aim of the paper was to examine the relation between bone density and certain parameters of lipid status in postmenopausal women. The research involved 300 women referred to densitometric examination as they belonged to the risk group of postmenopausal women. All the examinees had the following biochemical parameters determined: total cholesterol, triglycerides, HDL cholesterol, LDL cholesterol, glycemia, serum Ca and P. Univariate logistic regression analyses showed that each year of age, menopause duration, AH are significantly connected to risk increase for the appearance of osteopenia or osteoporosis. Increase in values of SBP, DBP, cholesterol, LDL and triglyceride are connected with significant risk increase for the appearance of osteopenia or osteoporosis. Patients with AH are connected to 11 times elevated risk for the appearance of osteopenia or osteoporosis, cigarette smoking increased the risk by seven times, physical inactivity even by 52 times, CVD in the family anamnesis by eight times, and osteoporosis in the family anamnesis is connected to the risk by four times. In our research, atherogenic lipoproteins negatively correlate with lumbar bone density. Disturbed lipide status is a risk factor for cardiovascular diseases, but also a risk factor for the appearance of osteoporosis.
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