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1

Production and application of doubled haploids in cereals

100%
Wedzony M.
Biotechnologia
|
2001
|
issue 1
51-62
EN
This paper provides an overview of the development of methods of cereals doubled haploid production over the last decade. The influence of genotype and albinism remain the main problem of androgenesis. Localisation of major genes influencing androgenic potential and manipulation with temperature during induction and regeneration offer possibilities of efficiency improvement. Isolated microspore culture in cereals is effective when microspores are co-cultured with sporophytic tissue. Gynogenic methods may be developed for some barley and apomictic wheat forms. Distant crosses that are followed by the elimination of chromosomes of the male parent from hybrid embryos are broadly applied. Maize and its relatives are used as effective pollinators of a wide range of cereal species since prezygotic barriers have not been found so far. Brief description of utilisation of doubled haploid lines in breeding programs, research and genetic transformation of cereals closes the overview.
2

The influence of various in vitro culture conditions on androgenetic embryo induction and plant regeneration from hexaploid triticale (x Triticosecale Wittm.)

39%
Ponitka A., Slusarkiewicz-Jarzina A., Wedzony M., Marcinska I., Wozny J., Wozna J.
Journal of Applied Genetics
|
1999
|
vol. 40
|
issue 3
165-174
EN
The effect of sucrose and maltose in culture media PII, C17 and MN6 on androgenetic embryo formation was investigated in seven F2 triticale hybrid progenies. In all genotypes, the highest number of androgenetic embryos (89.8-320.6/100 anthers) was formed on medium C17 containing maltose. Also the highest number of green plants (6.17/100 embryos) was regenerated from embryos obtained on PII with sucrose. The effect of the physical environment (temperature, light) in the first week of embryo culture on the regeneration medium was tested. The highest rate of green plants per 100 embryos (2.5-11.8) was obtained from incubation at 22oC in the dark. Key words: androgenetic embryos, incubation temperature, media, plant regeneration, triticale.
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