This work presents electrophoretic seed globulin data obtained fro 173 accessions representing nine Vicia species of the section Faba, whivh were already investigated for electrophoretic seed albumin patterns.Electrophoretic analysis of seed globulins was performed using sodium dodecyl suphate-polyacrylamide gel electrophoresis (SDS-PAGE).In the examined material totally 38 well-defined polypeptide bands, in the relative molecular mass range of 19-61 kD, were distinguished.The presence/absence of particular bands was recorded for each analysed individual.The electrophoretic data were used for hierarchical grouping of the examined taxa, based on calculated Bhattacharya distances.V bithynica proved to be the most distinctive member of Vicia section Faba.In turn, V.faba was clearly different from species of the V.narbonensis varieties and another - of the remaining species.In the latter group, V.serratifolia appeared to be rather distantly related to V.eristaliodes, V.galilaea, V.hyaeniscyamus, V.johannis and V.kalakhensis.The obtained results are compared with the corresponding seed albumin data and discussed with reference to taxonomic relatioships in Vicia section Faba.
The studied material covered 58 accessions representing Vicia faba of section Faba, V. bithynica of section Bithynicae and seven species of section Narbonensis. Proteins of individual seeds were separated by polyacrylamide gel electrophoresis and the gels were stained for inhibitory activity against trypsin. The number of trypsin inhibitor (TI) bands recorded in particular species varied from three in V. eristalioides and V. galilaea to 15 in V. narbonensis; in total, 30 bands were distinguished in the examined material. Except for V. eristalioides, the studied species showed intraspecific variation with respect to electrophoretic TI patterns. A statistical analysis of the data, using hierarchical UPGMA grouping, resolved the studied taxa into three main clusters. V. faba subspecies/varieties formed one cluster. The second cluster consisted of V. narbonensis varieties and the rather distantly related V. eristalioides. The third cluster comprised V. bithynica as a peripheral species and the more closely associated V. galilaea, V. hyaeniscyamus, V. johannis, V. kalakhensis and V. serratifolia. The obtained results are discussed with reference to taxonomic relationships of the species under study.
Electrophoretic analysis of seed albumins covered 201 accessions representing six subspecies of Vicia sativa L.: nigra, amphicarpa, incisa, sativa, macrocarpa and cordata. At least five individuals of each accession were examined separately. All the taxa under study showed variation with respect to albumin banding patterns, which was due to differences between accessions and individual variation within accessions. The number of albumin bands distinguished in particular taxa varied from 13 to 19. The statistical analysis of the electrophoretic data consisted in hierarchical grouping by the UPGMA method based on EUCLIDEAN distances. In the case of subsp. nigra and subsp. sativa, accessions originating from North Africa tended to form a group showing some distinctness from the remaining accessions. As to relationships among the studied taxa, subsp. nigra and subsp. cordata were shown to be the most closely related and their affinities to other members of the V. sativa aggregate were decreasing in the following order: subsp. macrocarpa, subsp. sativa, subsp. amphicarpa, subsp. incisa. The obtained results are discussed with reference to taxonomic relationships among the members of the V. sativa aggregate.
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